PDE1A active protein

PDE1A, Active

Cat. Num. AAA515627

• Gene Names: PDE1A; HCAM1; HCAM-1; HSPDE1A; CAM-PDE-1A
• Purity: >80%
• Synonyms: PDE1A; Active; PDE1A active protein

• Isotype: Sf9 cells
• Specificity: Full length protein expressed in Sf9 cells
• Purity/Purification: >80%
• Form/Format: Recombinant protein stored in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Concentration: 0.1 ug/ul (varies by lot)
• Sequence Length: 4918

• Specific Activity: 10,500 nmol/min/mg
• Preparation and Storage: Ships on dry ice. Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Specific Activity

(The specific activity of PDE1A was determined to be 10,500 nmol/min/mg as per activity assay protocol)

Puity

(The purity was determined to be >80% by densitometry. Approx. MW 64kDa.)

Related Product Information for PDE1A active protein

Description: Recombinant full-length human PDE1A was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Scientific Background: PDE1A is a member of the cyclic nucleotide phosphodiesterase family that is stimulated by Ca2+-calmodulin. PDE1A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5'-GMP. Calmodulin is tightly bound to PDE1A in human spermatozoa and this results in permanent activation of PDE1A (1). Inhibition of PDE1A function significantly attenuates vascular smooth muscle cell growth by decreasing proliferation via G1 arrest and induces apoptosis (2). In vascular smooth muscle cell, PDE1A is important in regulating growth and survival of these cells and thus can contribute to the neointima formation in atherosclerosis and restenosis.

Product Categories/Family for PDE1A active protein

Enzymes; Active Phosphodiesterases

References

1. Lefi¨vre L, et al: Presence of cyclic nucleotide phosphodiesterases PDE1A, existing as a stable complex with calmodulin, and PDE3A in human spermatozoa. Biol Reprod. 2002 Aug;67 (2):423-30. 2. Nagel D J, et al: Role of nuclear Ca2+/calmodulin-stimulated phosphodiesterase 1A in vascular smooth muscle cell growth and survival. Circ Res. 2006 Mar 31;98 (6):777-84.

NCBI and Uniprot Product Information

• NCBI GI #: 385198066
• NCBI GeneID: 5136
• NCBI Accession #: NM_005019.4
• NCBI GenBank Nucleotide #: NM_005019
• UniProt Accession #: P54750; Q86VZ0; Q9C0K8; Q9C0K9; Q9C0L0; Q9C0L1; Q9C0L2; Q9C0L3; Q9C0L4; Q9UFX3; D3DPG5
• Molecular Weight: ~64 kDa
• NCBI Official Full Name: Homo sapiens phosphodiesterase 1A (PDE1A), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: phosphodiesterase 1A
• NCBI Official Symbol: PDE1A
• NCBI Official Synonym Symbols: HCAM1; HCAM-1; HSPDE1A; CAM-PDE-1A
• NCBI Protein Information: calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
• UniProt Protein Name: Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
• Protein Family: Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase
• UniProt Gene Name: PDE1A
• UniProt Synonym Gene Names: Cam-PDE 1A

NCBI Description

Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]

Uniprot Description

PDE1A: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-GMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity