Rabbit anti-Human PCDHA9 Polyclonal Antibody | anti-PCDHA9 antibody

PCDHA9, NT (PCDHA9, KIAA0345, Protocadherin alpha-9) (Biotin)

Cat. Num. AAA6331530

• Gene Names: PCDHA9; PCDH-ALPHA9
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PCDHA9; Polyclonal Antibody; NT (PCDHA9; KIAA0345; Protocadherin alpha-9) (Biotin); Protocadherin alpha-9; anti-PCDHA9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-PCDHA9 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PCDHA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-261 amino acids from the N-terminal region of human PCDHA9.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PCDHA9 Antibody (N-term) western blot analysis in NCI-H460,K562 cell line lysates (35ug/lane).This demonstrates the PCDHA9 antibody detected the PCDHA9 protein (arrow).)

Flow Cytometry (FC/FACS)

(PCDHA9 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PCDHA9 antibody

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Product Categories/Family for anti-PCDHA9 antibody

Antibodies; Cadherin

References

Wu, C., et al. Proteomics 7(11):1775-1785(2007). Wu, Q., et al. Genome Res. 11(3):389-404(2001). Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000). Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000). Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 7662068
• NCBI GeneID: 9752
• NCBI Accession #: NP_054724.1; NP_114063.1
• NCBI GenBank Nucleotide #: NM_014005.4
• UniProt Accession #: Q9Y5H5; O15053; Q2M3S5
• Molecular Weight: 102,402 Da
• NCBI Official Full Name: protocadherin alpha-9 isoform 2
• NCBI Official Synonym Full Names: protocadherin alpha 9
• NCBI Official Symbol: PCDHA9
• NCBI Official Synonym Symbols: PCDH-ALPHA9
• NCBI Protein Information: protocadherin alpha-9; PCDH-alpha-9; KIAA0345-like 5
• UniProt Protein Name: Protocadherin alpha-9
• Protein Family: Protocadherin
• UniProt Gene Name: PCDHA9
• UniProt Synonym Gene Names: KIAA0345; PCDH-alpha-9
• UniProt Entry Name: PCDA9_HUMAN

Uniprot Description

PCDHA9: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: plasma membrane; integral to membrane

Molecular Function: calcium ion binding

Biological Process: homophilic cell adhesion