NCAM-L1 Active Protein | L1CAM active protein

NCAM-L1, Recombinant, Mouse (Neural Cell Adhesion Molecule L1, N-CAM-L1, NCAM-L1, CD171, L1cam, Caml1)

Cat. Num. AAA637838

• Gene Names: L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• Purity: Highly Purified; ~95% (SDS-PAGE, reducing conditions)
• Synonyms: NCAM-L1; Recombinant; Mouse (Neural Cell Adhesion Molecule L1; N-CAM-L1; CD171; L1cam; Caml1); L1CAM active protein

• Purity/Purification: Highly Purified; ~95% (SDS-PAGE, reducing conditions)
• Form/Format: Supplied as a lyophilized powder in MES, sodium chloride. Reconstitute with sterile PBS.

• Application Notes: SDS-PAGE: 180-200kD, reducing conditions
• Activity: Measured by the ability of the immobilized protein to support the adhesion of Neuro- 2a mouse neuroblastoma cells. When 5x10e4 cells/well are added to recombinant mouse NCAM-1/Fc Chimera coated plates (2.5ug/ml with 100ul/well), approximately 40%-60% will adhere after 50 minutes incubation at 37 degree C.
• Endotoxin Level: 1EU/1ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for L1CAM active protein

Neural cell adhesion molecule L1 (NCAM-L1; also L1-CAM, L1, and CD171) is a 200-220kD type I transmembrane glycoprotein of the immunoglobulin superfamily and L1/neurofascin/NgCAM family. NCAM-L1 is expressed by neurons, especially by growing axons on their growth cones. Non-neuronal cells such as Schwann cells, astrocytes, epithelial cells, and cells of myelomonocytic and lymphoid origin also express NCAM-L1. Full length mouse NCAM-L1 is synthesized as a 1260aa precursor that contains a 19aa signal sequence, a 1104aa extracellular domain (ECD), a 23aa transmembrane region, and a 114aa cytoplasmic tail that is highly conserved. The ECD contains six Ig-like C2-type domains, five fibronectin type III domains, and 21 potential sites for N-linked glycosylation. Mouse NCAM-L1 shares 88% aa sequence identity with human NCAM-L1. NCAM-L1 is critical for neural development. Specifically, NCAM-L1 plays a key role in neuronal cell migration, axon outgrowth, axon fasciculation, synaptogenesis, and myelination. NCAM-L1 mediates hemophilic cell-cell interaction but also binds heterophilic ligands like axonin-1, CD24, CD9, neurocan and several integrins. It has been shown that NCAM-L1 can undergo membrane-proximal cleavage by ADAM10 and ADAM17, leading to the release of the soluble extracellular domain and the generation of a membrane-bound stub. Remaining intact, the soluble extracellular domain has been suggested to serve as a substrate for integrin-mediated cell adhesion, thereby stimulating cellular motility and cell migration. It has also been found that NCAM-L1 plays a role in the ontogeny of human tumors and its expression is linked to poor prognosis. Overexpression promotes tumor-cell invasion and motility, growth in nude mice and tumor metastasis. Research shows that proteolytic processing by ADAM10 and presenilin/gamma-secretase is essential for the nuclear signaling of NCAM-L1 in human carcinoma cell lines. Defects in NCAM-L1 are the cause of MASA syndrome (mental retardation, aphasia, shuffling gait and adducted thumbs), which is also known as CRASH syndrome (corpus callosum hypoplasia, psychomotor retardation, adducted thumbs, spastic paraparesis and hydrocephalus.

Product Categories/Family for L1CAM active protein

Molecular Biology; MB-Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 497239609
• NCBI GeneID: 3897
• NCBI Accession #: NP_001265045.1
• NCBI GenBank Nucleotide #: NM_001278116.1
• UniProt Accession #: P32004; Q8TA87; A0AV65; A4ZYW4; B2RMU7; G3XAF4
• Molecular Weight: ~150kD
• NCBI Official Full Name: neural cell adhesion molecule L1 isoform 1
• NCBI Official Synonym Full Names: L1 cell adhesion molecule
• NCBI Official Symbol: L1CAM
• NCBI Official Synonym Symbols: S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• NCBI Protein Information: neural cell adhesion molecule L1; antigen identified by monoclonal antibody R1
• UniProt Protein Name: Neural cell adhesion molecule L1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: L1CAM
• UniProt Synonym Gene Names: CAML1; MIC5; N-CAM-L1; NCAM-L1
• UniProt Entry Name: L1CAM_HUMAN

NCBI Description

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]

Uniprot Description

NCAM-L1: cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. Two splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: presynaptic membrane; focal adhesion; cell surface; integral to membrane; plasma membrane; terminal button; external side of plasma membrane

Molecular Function: integrin binding; identical protein binding; protein self-association; sialic acid binding

Biological Process: nervous system development; axon guidance; positive regulation of calcium-mediated signaling; chemotaxis; heterophilic cell adhesion; leukocyte adhesion; cell surface receptor linked signal transduction; homotypic cell-cell adhesion; cell-cell adhesion mediated by integrin; positive regulation of cell-cell adhesion; cell adhesion; blood coagulation; homophilic cell adhesion; leukocyte migration

Disease: Hydrocephalus Due To Congenital Stenosis Of Aqueduct Of Sylvius; Corpus Callosum, Partial Agenesis Of, X-linked; Masa Syndrome