LRIG3 active protein

LRIG3, Recombinant, Human (Leucine-rich Repeats and Ig-like domains-3, LIG-3)

Cat. Num. AAA637520

• Gene Names: LRIG3; LIG3
• Purity: Highly Purified; 95% as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: LRIG3; Recombinant; Human (Leucine-rich Repeats and Ig-like domains-3; LIG-3); LRIG3 active protein

• Purity/Purification: Highly Purified; 95% as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS, pH 7.4.

• Activity: Measured by its ability to inhibit proliferation of the human cervical epithelium cancer cell line, Hela (Goldoni, S. et. al. 2007, Oncogene, 26:368-81). The ED50 for this effect is typically 1.5-6ug/ml.
• Endotoxin Level: < 1.0 EU per 1 g of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile phosphate-buffered saline be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months from date of receipt at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for LRIG3 active protein

LRIG3 (leucine-rich repeats and Ig-like domains-3) is a 140 kD type I transmembrane glycoprotein member of the mammalian LRIG glycoprotein family. This family contains three members who share 45-50% amino acid (aa) identity. 1 All members contain at least fifteen LRRs, accompanied by two flanking cysteine-rich regions, and three C2-type Ig-like domains in their extracellular domains (ECD). 1 LRIG3 mRNA is widely expressed, with highest levels in stomach, skin, thyroid and small intestine. 1 Human LRIG3 is synthesized as an 1120 amino acid (aa) precursor. It contains a 24 aa signal sequence, a 786 aa ECD, a 21 aa transmembrane sequence, and a 289 aa intracellular region. One splice variant exists that has a 19 aa substitution for the first 79 aa of the standard (or long) form. This substitution appears to encode an alternate signal sequence, resulting in a mature protein that lacks the first and part of the second LRR. LRIG1, a related family member, is known to bind the EGF family receptors ErbB1-4, via either its LRR or Ig-like domains. It also binds the ubiquitin ligase, c-Cbl, and promotes ubiquitination, internalization and destruction of these receptors. 2, 3 It is not known whether LRIG3 performs similar functions. Within the cell, LRIG3 is expressed in the perinuclear region as well as on the cell surface. Perinuclear location of LRIG3 in grade III and IV astrocytic tumors has been associated with better patient survival. 4 Human LRIG3 ECD shows 91%, 92%, 95% and 98% aa identity with mouse, rat, bovine and canine LRIG3 ECD, respectively.

Product Categories/Family for LRIG3 active protein

Molecular Biology; MB-Carbohydrates; Glycoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 209862903
• NCBI GeneID: 121227
• NCBI Accession #: NP_001129523.1
• NCBI GenBank Nucleotide #: NM_001136051.2
• UniProt Accession #: Q6UXM1; Q6UXL7; Q8NC72
• Molecular Weight: Based on N-terminal amino acid sequencing, the mature recombinant human LRIG-3 starts at Asp 28 and has a calculated molecular mass of approximately 87.1kD. As a result of glycosylation, the recombinant protein migrates as an approximately 105-115kD prote
• NCBI Official Full Name: leucine-rich repeats and immunoglobulin-like domains protein 3 isoform 1
• NCBI Official Synonym Full Names: leucine-rich repeats and immunoglobulin-like domains 3
• NCBI Official Symbol: LRIG3
• NCBI Official Synonym Symbols: LIG3
• NCBI Protein Information: leucine-rich repeats and immunoglobulin-like domains protein 3; LIG-3
• UniProt Protein Name: Leucine-rich repeats and immunoglobulin-like domains protein 3
• Protein Family: Leucine-rich repeats and immunoglobulin-like domains protein
• UniProt Gene Name: LRIG3
• UniProt Synonym Gene Names: LIG3; LIG-3
• UniProt Entry Name: LRIG3_HUMAN

Uniprot Description

LRIG3: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: extracellular space; cytoplasmic vesicle membrane; integral to membrane; plasma membrane

Biological Process: otolith morphogenesis