LMIR3 Active Protein
LMIR3, Recombinant, Human (CMRF35-like Molecule 1, CLM-1, CD300 Antigen-like Family Member F, Immune Receptor Expressed on Myeloid Cells 1, IREM-1, Immunoglobulin Superfamily Member 13, IgSF13, CD300f, NK Inhibitory Receptor, CD300LF, CD300F, CLM1, IGSF13
Purity
Highly Purified~95% (SDS-PAGE). Endotoxin Level: 1.0 EU/1ug (LAL).
Synonyms
LMIR3; Recombinant; Human (CMRF35-like Molecule 1; CLM-1; CD300 Antigen-like Family Member F; Immune Receptor Expressed on Myeloid Cells 1; IREM-1; Immunoglobulin Superfamily Member 13; IgSF13; CD300f; NK Inhibitory Receptor; CD300LF; CD300F; CLM1; IGSF13; LMIR3 active protein
Purity/Purification
Highly Purified
~95% (SDS-PAGE). Endotoxin Level: 1.0 EU/1ug (LAL).
~95% (SDS-PAGE). Endotoxin Level: 1.0 EU/1ug (LAL).
Form/Format
Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS.
Application Notes
SDS-PAGE: 57-62kD, reducing conditions
Activity
Measured by its ability to stimulate human T cell proliferation. The ED50 for this effect is typically 0.75-4.5ug/ml.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for LMIR3 active protein
LMIR3, also known as CD300LF, CD300F, IREM-1, CLM-1, IgSF13, DIgR1, and MAIR-V, is a 50-60kD glycoprotein member of the immunoglobulin superfamily. Human LMIR3 consists of a 137aa extracellular domain (ECD) with one Ig-like V-type domain, a 21aa transmembrane segment, and a 113aa cytoplasmic domain that contains multiple immunoreceptor tyrosine-based inhibitory motifs (ITIMs) or ITIM-like sequences. Alternate splicing generates additional isoforms that carry substituted C-terminal tails of varying lengths and sequences following the ECD or transmembrane segment. Within the ECD, human LMIR3 shares 43ª sequence identity with mouse and rat LMIR3. LMIR3 is expressed on the surface of dendritic cells, monocytes, granulocytes, and mast cells as well as on acute myeloid leukemia (AML) blasts. Pervanadate treatment or antibody cross-linking of LMIR3 induces phosphorylation of tyrosine residues in the cytoplasmic domain and the subsequent recruitment of phosphatases SHIP, SHP-1, SHP-2, and the p85 alpha subunit of PI3K. LMIR3 ligation can induce cell death and inhibit signaling through multiple receptors including Fc epsilon RI, LMIR4, SCF R, TLR2, TLR3, and TLR9. In contrast, it enhances TLR4-mediated signaling/cytokine production in mast cells through association with the activating signaling protein FcR gamma. In mouse, a splice variant of LMIR3 (known as DIgR2, with a 7aa insertion in the ECD) inhibits CD4+ T cell activation and in vivo Th1 and CTL responses. LMIR3 is upregulated on monocytes surrounding experimentally-induced spinal cord demyelination and functions as a negative regulator of inflammation in the CNS.
Product Categories/Family for LMIR3 active protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
42.4kD
NCBI Official Full Name
leukocyte mono-Ig-like receptor 3
Similar Products
Product Notes
The LMIR3 (Catalog #AAA636802) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. SDS-PAGE: 57-62kD, reducing conditions. Researchers should empirically determine the suitability of the LMIR3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LMIR3, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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