Lipase Active Protein

Lipase, Porcine Pancreatic

• Purity: Purified
• Synonyms: Lipase; Porcine Pancreatic; Triacylglycerol Acylhydrolase; Triacylglycerol lipase; PPL; Lipase (PPL); Porcine Pancreas - Lyophilized - High Purity; Lipase active protein

• Purity/Purification: Purified
• Form/Format: Lyophilized from saline with 3 mM calcium chloride, pH 7.0
• Sequence Length: 398

• Source: Porcine Pancreas
• EC Number: 3.1.1.3
• Unit Definition: One unit will catalyze the hydrolysis of one micromole per minute of 1, 2-0-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester in the presence of colipase, bile salt, and calcium chloride at 37 degree C.
• Specific Activity: 430 U/mg protein
• Reconstitution: >= 1 mg/mL in tris buffered saline, 1 mM calcium chloride, 1% BSA, pH 8.0 is recommended.
• Protein: 0.42 mg protein/mg solid (Coomassie)
• Contaminants: Amylase: < 0.01%; Cholesterol Esterase: < 0.01 %; Protease: < 0.01 %; Trypsin: 1.0%; Ammonia: < 0.01 micromole/mg
• CAS Number: 9001-62-1
• Activity: 180 U/mg (Dimension Clinical Chemistry System)
• Appearance: Off-white to tan powder
• Solubility: Clear, straw colored (10 mg/mL saline)
• Preparation and Storage: Aliquot and freeze at -20 degree C after reconstitution.; Protect from moisture.

Related Product Information for Lipase active protein

Custom preparations, technical support, bulk quantities and aliquoting available. Bulk quantities from 1-50 million unit single lot sizes. We are bulk manufacturers of human and animal pancreatic lipase for research and clinical manufacturers.

Lipase is a water soluble enzyme that catalyzes the hydrolysis of ester bonds in water insoluble, lipid substrates and is a glycoprotein. Lipase (triacylglycerol acyl hydrolase) fulfills a key function in dietary fat absorption by hydrolysing triglycerides into diglycerides and subsequently into monoglycerides and free fatty acids. Pancreatic enzymes have been most studied as part of the treatment for Celiac Disease.

Use: For investigation of pancreatic disorders, pancreatitis, acute and chronic renal disease.

Product Categories/Family for Lipase active protein

Proteins; Antigens; Enzymes; Standards/controls; Native Proteins; Lipase (ppl)

NCBI and Uniprot Product Information

• UniProt Accession #: P00591
• Protein Family: Lipase

NCBI Description

This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

LIPF: gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Protein type: Hydrolase; EC 3.1.1.3; Secreted; Lipid Metabolism - glycerolipid; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q23.31

Cellular Component: mitochondrion; extracellular region

Molecular Function: triacylglycerol lipase activity; malate dehydrogenase activity; lipid binding

Biological Process: malate metabolic process; triacylglycerol metabolic process; lipid catabolic process