Insulin-Like Growth Factor I Active Protein | IGF1 active protein

Insulin-Like Growth Factor I, Recombinant, Mouse (IGF1)

Cat. Num. AAA650619

• Gene Names: IGF1; IGFI; IGF-I; IGF1A
• Purity: Highly Purified; 98% by RP-HPLC, SDS-PAGE.
• Synonyms: Insulin-Like Growth Factor I; Recombinant; Mouse (IGF1); IGF1 active protein

• Purity/Purification: Highly Purified; 98% by RP-HPLC, SDS-PAGE.
• Form/Format: Supplied as a lyophilized powder. No additives. Reconstitute with ddH2O, 0.1% HSA or BSA to 100ug/ml, which can then be further diluted to other aqueous solutions.
• Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically.

• Biological Activity: IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is 1.0ng/ml, corresponding to a specific activity of 1x10e5 U/mg.
• Protein Content: Protein quantitation was carried out by two independent methods:; 1. UV spectroscopy at 280nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).; 2. Analysis by RP-HPLC, using a standard solution of IGF-I as a Reference Standard.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA and store at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for IGF1 active protein

A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor.

Recombinant murine IGF-1 is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6kD.

Product Categories/Family for IGF1 active protein

Growth Factors; Cytokines; Growth Factors-IGF

NCBI and Uniprot Product Information

• NCBI GI #: 184834
• NCBI GeneID: 3479
• UniProt Accession #: Q14620; P05019; Q13429; Q59GC5; Q5U743; Q6LD41; Q9NP10
• Molecular Weight: 7.6kD
• NCBI Official Full Name: insulin-like growth factor I
• NCBI Official Synonym Full Names: insulin-like growth factor 1 (somatomedin C)
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: IGFI; IGF-I; IGF1A
• NCBI Protein Information: insulin-like growth factor 1; MGF; IGF-IA; IGF-IB; somatomedin-C; OTTHUMP00000195080; OTTHUMP00000195081; OTTHUMP00000195082; OTTHUMP00000195083; OTTHUMP00000195084; mechano growth factor; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB
• UniProt Protein Name: Insulin-like growth factor 1 (Somatomedin C), isoform CRA_c
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: IGF1
• UniProt Entry Name: Q14620_HUMAN

NCBI Description

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

Uniprot Description

Subcellular location: Secreted

By similarity RuleBase RU000406.

Sequence similarities: Belongs to the insulin family. RuleBase RU000406