Western Blot (WB)
(ADP-ribosylation of PARP1 and HPF1. PARP1 is autoPARylated with the addition of 100uM NAD in the presence of activated DNA, lane B. When HPF1 is added, the autoPARylation profile shows lower MW staining, lane C. Also, HPF1 is ADP-ribosylated, lane C. The no NAD control shows no ADP-ribosylation, lane A. PARP1, HPF1, and autoPARylated PARP1 are indicated.)
HPF1 active protein
HPF1 protein, human
Synonyms
HPF1; HPF1 protein; human; Histone PARylation factor 1; C4orf27; HPF1 active protein
Form/Format
50ug HPF1 protein, human, lyophilized or 0.5mg (1mg/mL).
Western Blot (WB)
(ADP-ribosylation of PARP1 and HPF1. PARP1 is autoPARylated with the addition of 100uM NAD in the presence of activated DNA, lane B. When HPF1 is added, the autoPARylation profile shows lower MW staining, lane C. Also, HPF1 is ADP-ribosylated, lane C. The no NAD control shows no ADP-ribosylation, lane A. PARP1, HPF1, and autoPARylated PARP1 are indicated.)
Western Blot (WB)
(ADP-ribosylation of PARP1 and HPF1. PARP1 is autoPARylated with the addition of 100uM NAD in the presence of activated DNA, lane B. When HPF1 is added, the autoPARylation profile shows lower MW staining, lane C. Also, HPF1 is ADP-ribosylated, lane C. The no NAD control shows no ADP-ribosylation, lane A. PARP1, HPF1, and autoPARylated PARP1 are indicated.)
SDS-Page
SDS-Page
Related Product Information for HPF1 active protein
HPF1 protein, human, Cat. #2097, is highly purified and active recombinant full-length human HPF1, expressed in E. coli. The purified protein does not contain any expression tags. Please see the datasheet for additional information.
Mono- and poly-ADP-ribosylation are catalyzed by poly (ADP-ribose) polymerase (PARP) family proteins. PARP1/2 plays a critical role in the SSBR, and DSBR, the regulation of the stability of DNA replication forks, and maintenance of chromatin structures. Upon DNA damage, PARP1 rapidly recognizes and binds to a nicked or a gapped single-stranded DNA break which activates autoPARylation. Various PARP1-interacting regulatory factors play a role in the regulation of PARP1 activity. In response to DNA damage, histone PARylation factor 1 (HPF1 ; C4orf27) directly binds to the catalytic domain of PARP1/2 and promotes ADP-ribosylation at serine residues of PARP1, histone proteins, and other chromatin-associated factors. The serine-ADPr is a major and reversible post-translational modification in cells in response to DNA damage.
Mono- and poly-ADP-ribosylation are catalyzed by poly (ADP-ribose) polymerase (PARP) family proteins. PARP1/2 plays a critical role in the SSBR, and DSBR, the regulation of the stability of DNA replication forks, and maintenance of chromatin structures. Upon DNA damage, PARP1 rapidly recognizes and binds to a nicked or a gapped single-stranded DNA break which activates autoPARylation. Various PARP1-interacting regulatory factors play a role in the regulation of PARP1 activity. In response to DNA damage, histone PARylation factor 1 (HPF1 ; C4orf27) directly binds to the catalytic domain of PARP1/2 and promotes ADP-ribosylation at serine residues of PARP1, histone proteins, and other chromatin-associated factors. The serine-ADPr is a major and reversible post-translational modification in cells in response to DNA damage.
References
Gibbs-Seymour I, et al. (2016) Mol Cell. 62 432 doi.org/10.1016/j.molcel.2016.03.008
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Product Notes
The HPF1 (Catalog #AAA376044) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HPF1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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