G-CSF active protein

G-CSF, mouse

Cat. Num. AAA546277

• Gene Names: CSF3; GCSF; CSF3OS; C17orf33
• Purity: > 98% by SDS-PAGE and HPLC analyses.
• Synonyms: G-CSF; mouse; G-CSF active protein

• Purity/Purification: > 98% by SDS-PAGE and HPLC analyses.
• Form/Format: Lyophilized from a 0.2 um filtered concentrated solution in 10 mM Sodium Citrate, pH 4.0, 150 mM NaCI, 0.01 % Tween-20.
• Sequence: VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA

• Source: Escherichia coli
• Molecular Weight Information: Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 178 amino acids.
• Endotoxin: Less than 0.1 EU/ug of rMuG-CSF as determined by LAL method.
• Biological Activity: Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10⁷ IU/mg
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <= -20°C. Further dilutions should be made in appropriate buffered solutions.
• Preparation and Storage: This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree Cto -70 degree C.; Avoid repeated freeze/thaw cycles.

Related Product Information for G-CSF active protein

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-a and IFN-y. Besides, many other cell types can secreted this protein after LPS, IL-1 or TNF-a activation, which are fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition, it may function in some adhesion or recognition events at the cell surface. The murine G-CSF cDNA encodes a 208 amino acid (a.a.) residue precursor protein containing a 30 a.a. residue signal peptide that is proteolytically cleaved to generate the 178 a.a. residue mature protein. Murine G-CSF is 73 % identical at the amino acid level to human G-CSF and the two proteins show species cross-reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 743523204
• NCBI GeneID: 1440
• NCBI Official Full Name: granulocyte-colony stimulating factor, partial
• NCBI Official Synonym Full Names: colony stimulating factor 3
• NCBI Official Symbol: CSF3
• NCBI Official Synonym Symbols: GCSF; CSF3OS; C17orf33
• NCBI Protein Information: granulocyte colony-stimulating factor
• UniProt Protein Name: Granulocyte colony-stimulating factor
• UniProt Gene Name: CSF3
• UniProt Synonym Gene Names: C17orf33; GCSF; G-CSF
• UniProt Entry Name: CSF3_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.