Furin Mouse Active Protein | Furin active protein

Furin, Recombinant Mouse

Cat. Num. AAA635814

• Gene Names: Furin; Fur; PACE; SPC1; Pcsk3; 9130404I01Rik
• Purity: Highly Purified; ~95% (SDS-PAGE)
• Synonyms: Furin Mouse; Furin; Recombinant Mouse; Furin active protein

• Purity/Purification: Highly Purified; ~95% (SDS-PAGE)
• Form/Format: Supplied as a liquid in Tris, Calcium Chloride2, sodium chloride, Brij-35, Glycerol.

• Biological Activity: Measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC, The specific activity is >150 pmoles/min/ug, as measured under the described conditions.
• Endotoxin Level: <1EU/ug (LAL method)
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Furin active protein

Furin is a member of the proprotein convertase (PC) family, which belongs to the subtilisin superfamily of serine proteases. As a cellular protease, Furin processes a variety of proproteins in secretory pathway compartments by cleaving after ArgXaaLys/ArgArglike motifs, which usually reside at the end of the pro regions of these proproteins. Examples of the proprotein substrates are growth factors and receptors, extracellular matrix proteins, and other proteases. Furin has an essential role in embryogenesis and homeostasis and is implicated in various pathologies such as cancer, neurodegenerative diseases and anthrax (1, 2). Mouse Furin is a 793 amino acid type I transmembrane protein precursor with a signal peptide (residues 1 24), a pro region

Product Categories/Family for Furin active protein

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 125347022
• NCBI GeneID: 18550
• NCBI Accession #: NP_001074923.1
• NCBI GenBank Nucleotide #: NM_001081454.1
• UniProt Accession #: P23188; Q6GTN6
• Molecular Weight: ~77kD
• NCBI Official Full Name: furin preproprotein
• NCBI Official Synonym Full Names: furin (paired basic amino acid cleaving enzyme)
• NCBI Official Symbol: Furin
• NCBI Official Synonym Symbols: Fur; PACE; SPC1; Pcsk3; 9130404I01Rik
• NCBI Protein Information: furin; prohormone convertase 3; dibasic-processing enzyme; subtilisin pro-protein processing enzyme; proprotein convertase subtilisin/kexin type 3; paired basic amino acid residue cleaving enzyme; paired basic amino acid residue-cleaving enzyme
• UniProt Protein Name: Furin
• Protein Family: Furin
• UniProt Gene Name: Furin
• UniProt Synonym Gene Names: Fur; Pcsk3; PACE
• UniProt Entry Name: FURIN_MOUSE

NCBI Description

This gene encodes a calcium-dependent serine endoprotease that proteolytically activates different proprotein substrates traversing the secretory pathway. The encoded protein undergoes proteolytic autoactivation during which an N-terminal propeptide is cleaved to generate the mature protein. Mice lacking the encoded protein die at an embryonic stage and display hemodynamic insufficiency, cardiac ventral closure defect, axial rotation defect and abnormal yolk sac vasculature. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

FURIN: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Belongs to the peptidase S8 family. Furin subfamily.

Protein type: Membrane protein, integral; Cell surface; Vesicle; Protease; EC 3.4.21.75

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; Golgi cisterna; cell surface; early endosome; integral to membrane; trans-Golgi network; lipid raft; trans-Golgi network transport vesicle membrane; trans-Golgi network transport vesicle; membrane; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; peptidase activity; protein binding; protease binding; hydrolase activity; serine-type peptidase activity; metal ion binding; serine-type endopeptidase activity; endopeptidase activity; nerve growth factor binding; peptide binding

Biological Process: signal peptide processing; peptide hormone processing; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of signal transduction; proteolysis; peptide biosynthetic process; nerve growth factor production; negative regulation of transforming growth factor-beta1 production; cell proliferation; regulation of low-density lipoprotein receptor biosynthetic process; regulation of endopeptidase activity; regulation of protein catabolic process; protein processing; negative regulation of low-density lipoprotein receptor catabolic process; secretion by cell; positive regulation of cell migration