Folate Receptor 3 Active Protein | FOLR3 active protein

Folate Receptor 3, Recombinant, Human (FOLR3, Folate Receptor gamma)

Cat. Num. AAA636047

• Gene Names: FOLR3; FR-G; FR-gamma; gamma-hFR
• Purity: Highly Purified; 90% (SDS­PAGE). Endotoxin: 1EU/ug (LAL)
• Synonyms: Folate Receptor 3; Recombinant; Human (FOLR3; Folate Receptor gamma); FOLR3 active protein

• Purity/Purification: Highly Purified; 90% (SDS­PAGE). Endotoxin: 1EU/ug (LAL)
• Form/Format: Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS to 0.1mg/ml.

• Application Notes: SDS-PAGE: 30-40kD, reducing conditions. Predicted MW is 26kD.
• Biological Activity: Measured by its binding ability in a functional ELISA. When Folic Acid-BSA is coated at 5ug/ml (100ul/well), the concentration of rhFOLR-3 that produces 50% of the optimal binding response is found to be approximately 0.2-1nM.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for FOLR3 active protein

Folate Receptor 3 (FOLR3), also known as folate receptor gamma, is a 32kD protein that binds folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. Mature FOLR3 is a glycosylated protein that contains an imperfect carboxy terminal GPI anchor sequence. A fraction of FOLR3 is GPI-linked to the cell surface, but the majority is released as a secreted protein. Mature human FOLR3 shares 74% and 83% aa sequence identity with FOLR1 and FOLR2, respectively. Orthologs of human FOLR3 have not been described in mouse or rat. A genetic polymorphism generates a truncated isoform that lacks the carboxy terminal 139aa. FOLR3 is expressed by cells of the myelocyte and B lymphocyte lineage as well as by various carcinomas. FOLR3 is more highly expressed in ovarian carcinomas than in breast carcinomas or mesotheliomas. Unlike other folate receptors, FOLR3 does not exhibit stereoselectivity in the binding of folates.

Product Categories/Family for FOLR3 active protein

Molecular Biology; MB-Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 478887
• NCBI GeneID: 2352
• UniProt Accession #: P41439
• Molecular Weight: 26kD
• NCBI Official Full Name: folate receptor 3 (gamma)
• NCBI Official Synonym Full Names: folate receptor 3 (gamma)
• NCBI Official Symbol: FOLR3
• NCBI Official Synonym Symbols: FR-G; FR-gamma; gamma-hFR
• NCBI Protein Information: folate receptor gamma
• UniProt Protein Name: Folate receptor gamma
• Protein Family: Folate receptor
• UniProt Gene Name: FOLR3
• UniProt Synonym Gene Names: FR-gamma
• UniProt Entry Name: FOLR3_HUMAN

NCBI Description

This gene encodes a member of the folate receptor (FOLR) family, members of which have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This gene includes two polymorphic variants; the shorter one has two base deletion in the CDS, resulting in a truncated polypeptide, compared to the longer one. Both protein products are constitutively secreted in hematopoietic tissues and are potential serum marker for certain hematopoietic malignancies. The longer protein has a 71% and 79% sequence homology with the FOLR1 and FOLR2 proteins, respectively. [provided by RefSeq, Jul 2008]

Uniprot Description

FOLR3: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Isoform Short does not bind folate. Belongs to the folate receptor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extrinsic to membrane; membrane; extracellular region

Molecular Function: folic acid binding

Biological Process: folic acid transport