Fibroblast Growth Factor, Basic, Bovine Active Protein
Fibroblast Growth Factor, Basic, Recombinant, Bovine (FGFb)
Purity
Highly Purified97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Synonyms
Fibroblast Growth Factor; Basic; Bovine; Recombinant; Bovine (FGFb); Bovine active protein
Purity/Purification
Highly Purified
97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form/Format
Supplied as a lyophilized powder from 1% HSA. Reconstitute in sterile ddH2O to a concentration of >0.1mg/ml.
Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.
Biological Activity
rbFGF-b is fully biologically active when compared to standards. The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be less then 0.1ng/ml, corresponding to a specific activity of 3x10e6 Units/mg.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a standard solution of FGF-b as a Reference Standard.
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a standard solution of FGF-b as a Reference Standard.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Fibroblast Growth Factor, Basic, Bovine active protein
Single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor.
Recombinant Bovine FGF-b (FGF-2) is a single, non-glycosylated, polypeptide chain containing 155 amino acids having a molecular mass of 17,250D.
Recombinant Bovine FGF-b (FGF-2) is a single, non-glycosylated, polypeptide chain containing 155 amino acids having a molecular mass of 17,250D.
Product Categories/Family for Fibroblast Growth Factor, Basic, Bovine active protein
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Product Notes
The Fibroblast Growth Factor, Basic, Bovine (Catalog #AAA650532) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Al a-Gly-Ser. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor, Basic, Bovine, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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