EGF active protein

RECOMBINANT HUMAN EGF

Cat. Num. AAA232234

• Gene Names: EGF; URG; HOMG4
• Applications: ELISA, Functional Assay, Western Blot
• Purity: >98% by SDS PAGE and HPLC analysis
• Synonyms: EGF; RECOMBINANT HUMAN EGF; EGF active protein

• Purity/Purification: >98% by SDS PAGE and HPLC analysis
• Form/Format: Purified recombinant protein - lyophilised
• Concentration: Approximate Protein Concentration: 1.0 mg/ml after reconstitution (varies by lot)
• Sequence Length: 1166

• Applicable Applications for EGF active protein: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Recombinant human EGF may be used as athe standard in ELISA applications with either a purified human EGF antibody or a biotinylated human EGF antibody.; Western Blot: Recombinant human EGF may be used as the positive control for Wester Blotting application with either a purified human EGF antibody or a biotinylated human EGF antibody.; ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well; Functional Assays: Minimum Dilution: 0.5; Maximum Dilution: 25ng/ml; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
• Reconstitution: Reconstitute with 0.5ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended storage, the addition of 5% trehalose is recommended.; Preparation
• Activity: 1 x 107 units/mg
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for EGF active protein

Recombinant human epidermal growth factor is 6.2kDa globular protein comprosed of 53 amino acids. EGF is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 296011013
• NCBI GeneID: 1950
• NCBI Accession #: NP_001171601.1
• NCBI GenBank Nucleotide #: NM_001178130.1
• UniProt Accession #: P01133; Q52LZ6; B4DRK7; E7EVD2; E9PBF0
• Molecular Weight: Protein Molecular Weight: 6.2 kD (53 Amino acid sequence)
• NCBI Official Full Name: pro-epidermal growth factor isoform 2 preproprotein
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Official Synonym Symbols: URG; HOMG4
• NCBI Protein Information: pro-epidermal growth factor; beta-urogastrone
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: EGF
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hormone; Membrane protein, integral; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space; lysosomal membrane; integral to membrane; extracellular region; plasma membrane

Molecular Function: transmembrane receptor protein tyrosine kinase activator activity; protein binding; growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; transmembrane receptor protein tyrosine kinase activation (dimerization); nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; signal transduction; positive regulation of MAP kinase activity; platelet degranulation; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; angiogenesis; DNA replication; positive regulation of granule cell precursor proliferation; STAT protein nuclear translocation; positive regulation of DNA binding; epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of mitosis; branching morphogenesis of a tube; innate immune response; negative regulation of secretion; blood coagulation; positive regulation of phosphorylation

Disease: Hypomagnesemia 4, Renal