EG-VEGF, Active Active Protein | PROK1 active protein

EG-VEGF, Active, Recombinant, Human (Prokineticin-1, Endocrine-gland-derived Vascular Endothelial Growth Factor, Mambakine, PROK1, UNQ600/PRO1186)

Cat. Num. AAA650903

• Gene Names: PROK1; PK1; PRK1; EGVEGF
• Purity: Purified; ~95% (SDS-PAGE, HPLC). Endotoxin: 0.1ng/ug
• Synonyms: EG-VEGF; Active; Recombinant; Human (Prokineticin-1; Endocrine-gland-derived Vascular Endothelial Growth Factor; Mambakine; PROK1; UNQ600/PRO1186); PROK1 active protein

• Purity/Purification: Purified; ~95% (SDS-PAGE, HPLC). Endotoxin: 0.1ng/ug
• Form/Format: Supplied as a lyophilized powder. Reconstitute with sterile dH2O or PBS to 0.1-1mg/ml.

• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-5.0ng/ml.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for PROK1 active protein

Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. Study of tumor angiogenesis has led to the identification of several proteins including basic fibroblast growth factor (bFGF) and vascular endothelial growth factor. VEGF acts by interacting with a family of largely endothelial-specific receptor tyrosine kinases that includes VEGFR-1 (flt-1), VEGFR-2 (flk- 1/KDR), and VEGFR-3/Flt-4. Disruption of VEGFRs interferes with differentiation of endothelial cells and it is lethal for the embryo.

Product Categories/Family for PROK1 active protein

Growth Factors; Cytokines; Growth Factors-VEGF

NCBI and Uniprot Product Information

• NCBI GI #: 37182488
• NCBI GeneID: 84432
• UniProt Accession #: P58294; Q5VWD4; Q8TC69
• Molecular Weight: 9605 dalton
• NCBI Official Full Name: EG-VEGF
• NCBI Official Synonym Full Names: prokineticin 1
• NCBI Official Symbol: PROK1
• NCBI Official Synonym Symbols: PK1; PRK1; EGVEGF
• NCBI Protein Information: prokineticin-1; EG-VEGF; mambakine; OTTHUMP00000013289; black mamba toxin-related protein; endocrine-gland-derived vascular endothelial growth factor
• UniProt Protein Name: Prokineticin-1
• Protein Family: Prokineticin
• UniProt Gene Name: PROK1
• UniProt Entry Name: PROK1_HUMAN

NCBI Description

Endocrine gland-derived vascular endothelial growth factor (EG-VEGF) induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. Its expression is induced by hypoxia and is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta). Its expression is often complementary to the expression of VEGF (MIM 192240), suggesting that these molecules function in a coordinated manner.[supplied by OMIM]

Uniprot Description

Function: Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. Ref.1 Ref.2 Ref.9 Ref.10 Ref.11

Subcellular location: Secreted.

Tissue specificity: Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta. Ref.2 Ref.9 Ref.11

Developmental stage: In adult testis, is strongly expressed only in Leydig cells. In testicular tumors, expressed specifically in Leydig cell tumors (at protein level). Expressed from 14 weeks until birth in fetal testis.

Sequence similarities: Belongs to the AVIT (prokineticin) family.