Clostridium difficile Toxin B Active Protein
Clostridium difficile Toxin B, Recombinant
Purity
Highly Purified~95% (SDS-PAGE shows a band between 60-65kD under reducing conditions.) Endotoxin: <1EU/ug (LAL)
Synonyms
Clostridium difficile Toxin B; Recombinant; Clostridium difficile Toxin B active protein
Purity/Purification
Highly Purified
~95% (SDS-PAGE shows a band between 60-65kD under reducing conditions.) Endotoxin: <1EU/ug (LAL)
~95% (SDS-PAGE shows a band between 60-65kD under reducing conditions.) Endotoxin: <1EU/ug (LAL)
Form/Format
Supplied as a liquid in 25mM Tris base, 150mM sodium chloride, pH 7.5.
Biological Activity
Measured by its ability to hydrolyze UDPGlucose. The specific activity is > 45 pmoles/min/ug, as measured under the described conditions in the Activity Assay Protocol.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Clostridium difficile Toxin B active protein
Clostridium difficile is the leading cause of hospital-acquired diarrhea, known as C. difficile-associated disease. The estimated number of cases of C. difficile-associated disease exceeds 250,000 per year (1), with health care costs approaching US $1 billion annually (2).
The major virulence factors produced by C. difficile are two toxins, TcdA and TcdB. Both toxins can monoglucosylate and inactivate Rho family small GTPases within target cells, leading to disruption of vital signaling pathways in the cell, subsequently causing diarrhea, inflammation, and damage of colonic mucosa (3,4,5). Both toxins have a similar tripartite structure comprised of an N-terminal glucosyltransferase domain, a C-terminal receptor binding domain, and a small hydrophobic span possibly involved in toxin translocation (6).
Our recombinant TcdB consists of the enzymatic domain. Both TcdA and TcdB also have potassium-dependent UDPGlc hydrolase activity, which is essentially glucosyltransferase activity with water as the acceptor molecule (7). Under the same conditions, UDP-glucose hydrolysis by TcdB occurs at a rate about 5-fold greater than that of TcdA.
The major virulence factors produced by C. difficile are two toxins, TcdA and TcdB. Both toxins can monoglucosylate and inactivate Rho family small GTPases within target cells, leading to disruption of vital signaling pathways in the cell, subsequently causing diarrhea, inflammation, and damage of colonic mucosa (3,4,5). Both toxins have a similar tripartite structure comprised of an N-terminal glucosyltransferase domain, a C-terminal receptor binding domain, and a small hydrophobic span possibly involved in toxin translocation (6).
Our recombinant TcdB consists of the enzymatic domain. Both TcdA and TcdB also have potassium-dependent UDPGlc hydrolase activity, which is essentially glucosyltransferase activity with water as the acceptor molecule (7). Under the same conditions, UDP-glucose hydrolysis by TcdB occurs at a rate about 5-fold greater than that of TcdA.
Product Categories/Family for Clostridium difficile Toxin B active protein
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Product Notes
The Clostridium difficile Toxin B (Catalog #AAA635992) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Clostridium difficile Toxin B, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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