CLEC9A active protein

CLEC9A, Recombinant, Human (C-type Lectin Domain Family 9 Member A, UNQ9341/PRO34046)

Cat. Num. AAA635830

• Gene Names: CLEC9A; DNGR1; UNQ9341
• Purity: Highly Purified; ~95% (SDS-PAGE, reducing conditions)
• Synonyms: CLEC9A; Recombinant; Human (C-type Lectin Domain Family 9 Member A; UNQ9341/PRO34046); CLEC9A active protein

• Purity/Purification: Highly Purified; ~95% (SDS-PAGE, reducing conditions)
• Form/Format: Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS.

• Application Notes: SDS-PAGE: 55-65kD, reducing conditions
• Activity: Measured by flow cytometry for its ability to bind a ligand expressed in FasL-treated mouse B cells. At 100ng/ml, 50% of 7AAD positive cells bind to rhCLEC9a.
• Endotoxin Level: 1EU/1ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CLEC9A active protein

CLEC9a (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily. Mature human CLEC9a consists of a 35aa cytoplasmic domain with an ITAM-like motif, a 21aa transmembrane segment, and a 185aa extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD). Within the ECD, human CLEC9a shares 57% aa sequence identity with mouse and rat CLEC9a. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of approximately 50kD N-glycosylated subunits. Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16-monocytes. BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk.

Product Categories/Family for CLEC9A active protein

Lectins; Lectin Other

NCBI and Uniprot Product Information

• NCBI GI #: 46409326
• NCBI GeneID: 283420
• NCBI Accession #: NP_997228.1
• NCBI GenBank Nucleotide #: NM_207345.2
• UniProt Accession #: Q6UXN8; B0ZBM2
• Molecular Weight: ~47kD
• NCBI Official Full Name: C-type lectin domain family 9 member A
• NCBI Official Synonym Full Names: C-type lectin domain family 9, member A
• NCBI Official Symbol: CLEC9A
• NCBI Official Synonym Symbols: DNGR1; UNQ9341
• NCBI Protein Information: C-type lectin domain family 9 member A; HEEE9341
• UniProt Protein Name: C-type lectin domain family 9 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC9A
• UniProt Entry Name: CLC9A_HUMAN

NCBI Description

CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells (Huysamen et al., 2008 [PubMed 18408006]).[supplied by OMIM, Aug 2008]

Uniprot Description

CLEC9A: Functions as an endocytic receptor on a small subset of myeloid cells and induces intracellular signaling via spleen tyrosine kinase (Syk). Acts as a receptor for necrotic cells and can mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: cell surface; integral to membrane

Molecular Function: carbohydrate binding

Biological Process: receptor-mediated endocytosis; positive regulation of cytokine secretion