CD160, 6-His tag Active Protein
CD160, Recombinant, Mouse, 6-His tag
Purity
Purified~95% (SDS-PAGE)
Synonyms
CD160; 6-His tag; Recombinant; Mouse; 6-His tag active protein
Purity/Purification
Purified
~95% (SDS-PAGE)
~95% (SDS-PAGE)
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitute with PBS 250ug/ml.
Biological Activity
Measured by its binding ability in a functional ELISA. When recombinant mouse HVEM Fc Chimera is immobilized at 0.5ug/ml (100ul/well), the concentration of recombinant mouse CD160 that produces 50% of the optimal binding response is found to be ~0.012-0.06ug/ml.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for CD160, 6-His tag active protein
CD160 (also Natural killer cell receptor BY55) is a 16kD (predicted) member of the Ig superfamily (1-4). In mouse, it is expressed principally on nonmyeloid hematopoietic cells. These include CD3+ NK1.1 cells, CD8+ TEM and TCM T cells, CD8a+ IELs, NKT cells, CD8-yd TCR T cells, and vascular endothelial cells (1, 5-7). Mouse CD160 has been identified as a 20-21kD GPI-linked glycoprotein (4, 5). It is synthesized as a preproprotein that is 185aa in length. The precursor contains a 27 aa signal sequence, a 133 aa mature molecule that shows one 98 aa V-type Ig-like domain (aa 28-125), and a 25 aa prosegment that is cleaved to generate a GPI-linkage at Ser160. Mouse GPI-linked CD160 is known to be cleaved by phospholipase C, and this generates a 40kD (presumably dimeric) band in SDS-PAGE (5). One alternative splice form for mouse CD160 is reported that appears to show a deletion of aa 137 - 180. This may generate a soluble molecule (5; GenBank Accession # NP_001156969). Mature mouse CD160 shares 63% and 88% aa identity with human and rat CD160, respectively. In mouse, CD160 is reported to bind to bind to HVEM/TNFRSF14, and both classical and non-classical MHC Class I molecules (5, 8). MHC-I proteins recognized by CD160 include Dd, Kb, Qa-1b and CD1d (5). Upon engagement, the effects of CD160 ligation appear to be context dependent. When expressed on endothelial cells, CD160 binding to human HLA-G1 initiates apoptosis, and thus impacts angiogenesis (6). When expressed on NK1.1 cells, mouse CD160 ligation alone has no effect; when combined with NK1.1 antigen stimulation, CD160 decreases NK cell IFN-y secretion. Relative to cytotoxicity, NK cell activity is positively correlated with the presence of CD160 (5).
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Product Notes
The CD160, 6-His tag (Catalog #AAA636939) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CD160, 6-His tag, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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