CD11c Active Protein | CD83 active protein

CD11c, Recombinant, Human (Monocyte/Macrophage, CD11 Antigen-like Family Member C, Complement Component 3 Receptor 4 Subunit, Integrin alphaX, ITGAX, ITAX, Leukocyte Adhesion Glycoprotein p150,95 alpha Chain, Leukocyte Adhesion Receptor p150,95, Leu M5, L

Cat. Num. AAA637243

• Gene Names: CD83; BL11; HB15
• Purity: Purified; 95% (SDS­PAGE)
• Synonyms: CD11c; Recombinant; Human (Monocyte/Macrophage; CD11 Antigen-like Family Member C; Complement Component 3 Receptor 4 Subunit; Integrin alphaX; ITGAX; ITAX; Leukocyte Adhesion Glycoprotein p150; 95 alpha Chain; Leukocyte Adhesion Receptor p150; 95; Leu M5; L; CD83 active protein

• Purity/Purification: Purified; 95% (SDS­PAGE)
• Form/Format: Supplied as a lyophilized powder from 0.2um filtered solution in PBS. Reconstitute with sterile 200ug/ml PBS.

• Application Notes: SDS-PAGE: 149 & 103kD, reducing conditions.
• Biological Activity: Measured by the ability of immobilized protein to support adhesion of J45.01 human acute T cell lymphoblastic leukemia cells. When 5X10e4 cells are added to rhIntegrin alphaXbeta2 coated plates (10ug/ml, 100ul/well), ~ 50%-80% will adhere after 1 hour at 37 degree C.
• Endotoxin Level: 1EU/ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CD83 active protein

CD11c antigen is expressed mainly on monocytes and in low density on granulocytes and NK cells. High levels of CD11c are expressed on tissue macrophages. It is also found on lymphoid cell lines including hairy cell leukemia. CD11c (integrin aX subunit) combines with CD18 (integrin b2 subunit) to form the integrin protein 150,95 (aXb2, CD11c/CD18). CD11c/CD18 has been reported to play an important role in cytotoxic T cell killing, and in neutrophil and monocyte adhesion to endothelium. Although its ligands in these two cases have not been identified. CD11c/CD18 is not expressed on leukocytes of patients with leukocyte adhesion deficiency. Leukocyte adhesion deficiency results from defects in the CD18 gene, leading to diminished expression of all CD18-containing integrins. CD11c antibodies bind multiple ligands including the complement fragment iC3b, CD54 (ICAM-1), fibrinogen and bacterial lippolysaccharide.

Product Categories/Family for CD83 active protein

Molecular Biology; MB-CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 353677976
• NCBI GeneID: 9308
• NCBI Accession #: NP_001238830.1
• NCBI GenBank Nucleotide #: NM_001251901.1
• UniProt Accession #: Q01151; Q5THX9
• Molecular Weight: 23,042 Da
• NCBI Official Full Name: CD83 antigen isoform c
• NCBI Official Synonym Full Names: CD83 molecule
• NCBI Official Symbol: CD83
• NCBI Official Synonym Symbols: BL11; HB15
• NCBI Protein Information: CD83 antigen; hCD83; B-cell activation protein; cell surface protein HB15; cell-surface glycoprotein; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)
• UniProt Protein Name: CD83 antigen
• Protein Family: CD83 antigen
• UniProt Gene Name: CD83
• UniProt Synonym Gene Names: hCD83
• UniProt Entry Name: CD83_HUMAN

NCBI Description

The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

CD83: May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Biological Process: negative regulation of interleukin-4 production; positive regulation of interleukin-2 production; defense response; positive regulation of CD4-positive, alpha beta T cell differentiation; signal transduction; response to organic cyclic substance; humoral immune response; positive regulation of interleukin-10 production