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Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD11c (BU15) Pacific Blue™.)

Mouse CD11c Monoclonal Antibody | anti-CD11c antibody

Anti-Hu CD11c Pacific Blue

Gene Names
ITGAX; CD11C; SLEB6
Reactivity
Human, Monkey
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD11c; Monoclonal Antibody; Anti-Hu CD11c Pacific Blue; Integrin alpha X; ITGAX; SLEB6; integrin subunit alpha X; anti-CD11c antibody
Ordering
Host
Mouse
Reactivity
Human, Monkey
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
BU15
Specificity
The antibody BU15 reacts with an extracellular epitope of CD11c (alphaX, p150), a 150kDa integrin expressed mainly on dendritic cells and tissue macrophages.
Purity/Purification
The purified antibody is conjugated with Pacific Blue under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Pacific Blue. Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Applicable Applications for anti-CD11c antibody
Flow Cytometry (FC/FACS) (QC tested)
Application Notes
FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Immunogen
Dendritic cells of synovial fluid
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD11c (BU15) Pacific Blue™.)

Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-CD11c (BU15) Pacific Blue™.)
Related Product Information for anti-CD11c antibody
CD11c (p150, alphaX integrin subunit) forms complex with CD18 (beta2 integrin subunit) and is expressed mainly on tissue macrophages and dendritic cells. CD11c binds to complement fragment iC3b, fibrinogen, VCAM-1 and ICAM-2 or e.g. CD90. Like other beta2 integrins, CD11c/CD18 plays roles in cell migration and phagocytosis. Moreover, interaction of CD11c/CD18 with plasminogen regulates plasmin activities, and interaction with heparin counteracts binding of iC3b.
References
*Van der Vieren M, Le Trong H, Wood CL, Moore PF, St John T, Staunton DE, Gallatin WM: A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM-3. Immunity. 1995 Dec;3(6):683-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/8777714ordinal,*Myou S, Zhu X, Boetticher E, Qin Y, Myo S, Meliton A, Lambertino A, Munoz NM, Hamann KJ, Leff AR: Regulation of adhesion of AML14.3D10 cells by surface clustering of beta2-integrin caused by ERK-independent activation of cPLA2. Immunology. 2002 Sep;107(1):77-85., URL: http://www.ncbi.nlm.nih.gov/pubmed/12225365ordina,*Sadhu C, Hendrickson L, Dick KO, Potter TG, Staunton DE: Novel Tools for Functional Application (FUNC)tional Analysis of CD11c: Activation-Specific, Activation-Independent, and Activating Antibodies. J Immunoassay Immunochem. 2008;29(1):42-57., URL: http://www.ncbi.nlm.nih.gov/pubmed/18080879ordina,*Angel CE, Lala A, Chen CJ, Edgar SG, Ostrovsky LL, Dunbar PR: CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts. Int Immunol. 2007 Nov;19(11):1271-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/17804688itool=,*Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP-1alpha) and MIP-1beta production in primary human monocytes through a pathway dependent on nuclear factor-kappaB. Blood. 2001 May 15;97(10):2932-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/11342414ordina,*Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987)., URL:,*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995)., URL:,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL:,*Hogg N, Takacs L, Palmer DG, Selvendran Y, Allen C: The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules. Eur J Immunol. 1986 Mar;16(3):240-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/3456894ordinal,*Trowald-Wigh G, Johannisson A, Hakansson L: Canine neutrophil adhesion proteins and Fc-receptors in healthy dogs and dogs with adhesion protein deficiency, as studied by flow cytometry. Vet Immunol Immunopathol. 1993 Oct;38(3-4):297-310., URL: http://www.ncbi.nlm.nih.gov/pubmed/8291207ordinal
*Vorup-Jensen T, Chi L, Gjelstrup LC, Jensen UB, Jewett CA, Xie C, Shimaoka M, Linhardt RJ, Springer TA: Binding between the integrin alphaXbeta2 (CD11c/CD18) and heparin. J Biol Chem. 2007 Oct 19;282(42):30869-77., URL: http://www.ncbi.nlm.nih.gov/pubmed/17699512ordina,*Gang J, Choi J, Lee JH, Nham SU: Identification of critical residues for plasminogen binding by the alphaXI-domain of the beta2 integrin, alphaXbeta2. Mol Cells. 2007 Oct 31;24(2):240-6., URL: http://www.ncbi.nlm.nih.gov/pubmed/17978577ordina,*Sadhu C, Ting HJ, Lipsky B, Hensley K, Garcia-Martinez LF, Simon SI, Staunton DE: CD11c/CD18: novel ligands and a role in delayed-type hypersensitivity. J Leukoc Biol. 2007 Jun;81(6):1395-403., URL: http://www.ncbi.nlm.nih.gov/pubmed/17389580ordina,*Bullard DC, Hu X, Adams JE, Schoeb TR, Barnum SR: p150/95 (CD11c/CD18) expression is required for the development of experimental autoimmune encephalomyelitis. Am J Pathol. 2007 Jun;170(6):2001-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/17525267ordina

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
150kDa
NCBI Official Full Name
integrin alpha-X
NCBI Official Synonym Full Names
integrin, alpha X (complement component 3 receptor 4 subunit)
NCBI Official Symbol
ITGAX
NCBI Official Synonym Symbols
CD11C; SLEB6
NCBI Protein Information
integrin alpha-X; leu M5, alpha subunit; p150 95 integrin alpha chain; CD11 antigen-like family member C; leukocyte adhesion receptor p150,95; myeloid membrane antigen, alpha subunit; leukocyte surface antigen p150,95, alpha subunit; leukocyte adhesion gl
UniProt Protein Name
Integrin alpha-X
UniProt Gene Name
ITGAX
UniProt Synonym Gene Names
CD11C
UniProt Entry Name
ITAX_HUMAN

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Product Notes

The CD11c itgax (Catalog #AAA1802168) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD11c Pacific Blue reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD11c can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS) (QC tested). FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Researchers should empirically determine the suitability of the CD11c itgax for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11c, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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