Caspase 4, Active Active Protein | CASP10 active protein

Caspase 4, Active, Recombinant, Human (ICErelII, TX, Ich-2)

Cat. Num. AAA635796

• Gene Names: CASP10; MCH4; ALPS2; FLICE2
• Purity: Molecular Biology Grade
• Synonyms: Caspase 4; Active; Recombinant; Human (ICErelII; TX; Ich-2); CASP10 active protein

• Purity/Purification: Molecular Biology Grade
• Form/Format: Supplied as a lyophilized powder.

• Application Notes: Useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.; Dilution: 1 unit per assay for analyzing caspase activity.
• Specific Activity: ~5000 units/mg
• Reconstitution: Reconstitute to 1 unit per ml in PBS.
• Unit Definition: One unit of the recombinant caspase-4 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37 degree C in a reaction solution containing 50mM Hepes, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% Glycerol, 10mM DTT.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. For long term storage, store at -70 degree C for 1 year. To avoid multiple freeze thaw cycles, reconstituted product should be aliquoted and stored at 70 degree C. Aliquots are stable for 12 months at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CASP10 active protein

Caspase-4 (also know as ICErelII, TX, Ich-2) is the member of the caspase family of cysteine proteases. Caspase-4 exists in cells as an inactive pro-enzyme. The pro-enzyme is matured by proteolysis to yield large and small subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of caspase-4 activation and roles of caspase-4 in apoptosis are poorly understood. Active recombinant human caspase-4 was expressed in E. coli. The expressed caspase-4 spontaneously undergoes auto-processing to yield subunits caracteristic of the native enzyme. The active caspase-4 is routinely tested at Untied States Biological for its ability to enzymatically cleaves the substrate WEHD-AFC or WEHD-pNA.

Product Categories/Family for CASP10 active protein

Molecular Biology; MB-Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 14916490
• NCBI GeneID: 843
• NCBI Accession #: NP_116758.1
• NCBI GenBank Nucleotide #: NM_032976.3
• UniProt Accession #: Q92851; Q68HC0; Q6KF62; Q6KF63; Q8IUP5; Q8WYQ8; Q99845; Q9Y2U6; Q9Y2U7
• Molecular Weight: 58,951 Da
• NCBI Official Full Name: caspase-10 isoform 4
• NCBI Official Synonym Full Names: caspase 10, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP10
• NCBI Official Synonym Symbols: MCH4; ALPS2; FLICE2
• NCBI Protein Information: caspase-10; CASP-10; FADD-like ICE2; apoptotic protease MCH-4; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; caspase 10, apoptosis-related cysteine protease; FAS-associated death domain protein interleukin-1B-converting enzyme 2
• UniProt Protein Name: Caspase-10
• Protein Family: Caspase
• UniProt Gene Name: CASP10
• UniProt Synonym Gene Names: MCH4; CASP-10; FLICE2
• UniProt Entry Name: CASPA_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

CASP10: Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 23/17 kDa (p23/17) (depending on the splicing events) and a 12 kDa (p12) subunit. Self-associates. Interacts with FADD and CASP8. Found in a Fas signaling complex consisting of FAS, FADD, CASP8 and CASP10. Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon. Belongs to the peptidase C14A family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.63; Protease

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: cytoplasm; plasma membrane; CD95 death-inducing signaling complex; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; cysteine-type endopeptidase activity

Biological Process: regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; innate immune response; proteolysis

Disease: Lymphoma, Non-hodgkin, Familial; Gastric Cancer; Autoimmune Lymphoproliferative Syndrome, Type Iia