Carboxypeptidase A4 Active Protein | CPA4 active protein

Carboxypeptidase A4, Recombinant, Human (CPA4)

Cat. Num. AAA637050

• Gene Names: CPA4; CPA3
• Purity: Purified; 95% (SDS-PAGE)
• Synonyms: Carboxypeptidase A4; Recombinant; Human (CPA4); CPA4 active protein

• Purity/Purification: Purified; 95% (SDS-PAGE)
• Form/Format: Supplied as a liquid in TRIS, sodium chloride. Carrier free.

• Specific Activity: >3,500pmoles/min/ug
• Biological Activity: Measured by its ability to cleave the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5, 5'Dithio-bis (2-nitrobenzoic acid) (DTNB).
• Endotoxin Level: 1EU/ug (LAL)
• Preparation and Storage: Aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Related Product Information for CPA4 active protein

Carboxypeptidase A4, al so known as CPA4, is a secreted, zinc-dependent metallocarboxypeptidase that removes the C-terminal amino acid from peptides having a free C-terminal carboxyl group. CPA4 can hydrolyze both amide and ester bonds and has a preference for cleavage at the amino side of hydrophobic residues. CPA4 is inhibited by latexin, an endogenous inhibitor of carboxypeptidases. The deduced aa sequence of human CPA4 consists of a signal peptide (residues 1-16), a pro region (17-113), and a mature chain (residues 114-421). The CPA4 gene has been associated with prostate cancer aggressiveness and is involved in the histone hyperacetylation signaling pathway. Recent studies show that coding variation in the CPA4 gene is linked to high risk prostate cancer among younger patients.

Product Categories/Family for CPA4 active protein

Molecular Biology; MB-Enzymes; Carboxypeptidase

NCBI and Uniprot Product Information

• NCBI GI #: 61743916
• NCBI GeneID: 51200
• NCBI Accession #: NP_057436.2
• NCBI GenBank Nucleotide #: NM_016352.3
• UniProt Accession #: Q9UI42; Q86UY9; B7Z576
• Molecular Weight: 48kD
• NCBI Official Full Name: carboxypeptidase A4 preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase A4
• NCBI Official Symbol: CPA4
• NCBI Official Synonym Symbols: CPA3
• NCBI Protein Information: carboxypeptidase A4; carboxypeptidase A3
• UniProt Protein Name: Carboxypeptidase A4
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPA4
• UniProt Synonym Gene Names: CPA3
• UniProt Entry Name: CBPA4_HUMAN

NCBI Description

This gene is a member of the carboxypeptidase A/B subfamily, and it is located in a cluster with three other family members on chromosome 7. Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. This gene could be involved in the histone hyperacetylation pathway. It is imprinted and may be a strong candidate gene for prostate cancer aggressiveness. [provided by RefSeq, Jul 2008]

Uniprot Description

CPA4: Metalloprotease that could be involved in the histone hyperacetylation pathway. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Protease; EC 3.4.17.-

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: extracellular region

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: histone acetylation; proteolysis