ANGPTL5, 6-His Tag Active Protein
ANGPTL5, Recombinant, Human, 6-His Tag (Angiopoietin-related Protein 5, Angiopoietin-like Protein 5, UNQ5795/PRO19600)
Purity
Purified~90% (SDS-PAGE)
Synonyms
ANGPTL5; 6-His Tag; Recombinant; Human; 6-His Tag (Angiopoietin-related Protein 5; Angiopoietin-like Protein 5; UNQ5795/PRO19600); 6-His Tag active protein
Purity/Purification
Purified
~90% (SDS-PAGE)
~90% (SDS-PAGE)
Form/Format
Supplied as a lyophilized powder in PBS, sodium chloride, BSA. Reconstitute with PBS to 100ug/ml.
Biological Activity
Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of recombinant mouse SCF, recombinant mouse Thrombopoietin and recombinant mouse Flt-3 Ligand. The ED50 for this effect is typically 70-280ng/ml in the presence of a cross-linking mouse polyHistidine antibody.
Endotoxin Level
1EU/1ug (LAL)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for ANGPTL5, 6-His Tag active protein
Angiopoietin-like 5 (ANGPTL5) is a secreted glycoprotein that is structurally related to the angiopoietins which contain an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (1-3). It shares 88% and 93% aa sequence identity with bovine and porcine ANGPTL5, and greater than 98% identity with chimpanzee, orangutan, and rhesus ANGPTL5. ANGPTL5 is expressed in adipose, bronchial, epididymal, and heart tissue (3, 4). Rare polymorphisms and loss of function mutations in human ANGPTL5 are associated with low circulating triglyceride levels and alterations in body mass index (4, 5). ANGPTL5, when used in combination with other growth factors such as SCF, Thrombopoietin, IGF-II, FGF acidic, Flt-3 Ligand, and IGFBP-2, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (6-8). The coiled-coil domain of ANGPTL proteins is critical for this effect. However, the molecular mechanism of ANGPTL proteins in stem cell activity remains unclear (6).
Product Categories/Family for ANGPTL5, 6-His Tag active protein
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Product Notes
The ANGPTL5, 6-His Tag (Catalog #AAA636025) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ANGPTL5, 6-His Tag, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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