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Western Blot (Western bolt analysis of beta-Actin in 293 cells. Twenty micrograms of total cell lysates were bloted with mouse anti-beta-actin mouse monoclonal antibody (G043).)

beta-actin Antibody | anti-ACTB antibody

beta-actin antibody

Reactivity
Human, Bovine, Sheep, Pig, Rabbit, Feline, Dog, Mouse, Rat, Guinea Pig, Chicken, Carp
Applications
Western Blot
Synonyms
beta-actin; beta-actin antibody; anti-ACTB antibody
Ordering
For Research Use Only!
Reactivity
Human, Bovine, Sheep, Pig, Rabbit, Feline, Dog, Mouse, Rat, Guinea Pig, Chicken, Carp
Clone Number
G043
Specificity
Recognize 43KDa beta-actin from Human
Form/Format
1mg/ml in PBS, pH 7.4 with 0.05% sodium azide. Frozen liquid.
Sequence Length
159
Applicable Applications for anti-ACTB antibody
Western Blot (WB)
Application Notes
WB: 1:1000-4000
Immunogen
Beta-cytoplasmic actin N-terminal peptide.
Preparation and Storage
Stable for 1 year at-20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4 degree C for 3 months.

Western Blot

(Western bolt analysis of beta-Actin in 293 cells. Twenty micrograms of total cell lysates were bloted with mouse anti-beta-actin mouse monoclonal antibody (G043).)

Western Blot (Western bolt analysis of beta-Actin in 293 cells. Twenty micrograms of total cell lysates were bloted with mouse anti-beta-actin mouse monoclonal antibody (G043).)
References
Lee CS, et al. (2004) Exp Mol Med, 36 (6):582-587. McElhinny AS, et al. (2005) J Cell Biol, 170 (6): 947-957.

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
17,739 Da
NCBI Official Full Name
beta-actin, partial
UniProt Protein Name
Beta-actin
Protein Family

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Product Notes

The ACTB (Catalog #AAA850142) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The beta-actin antibody reacts with Human, Bovine, Sheep, Pig, Rabbit, Feline, Dog, Mouse, Rat, Guinea Pig, Chicken, Carp and may cross-react with other species as described in the data sheet. AAA Biotech's beta-actin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-4000. Researchers should empirically determine the suitability of the ACTB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-actin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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