Immunohistochemistry (IHC)
(Biotinylated (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Aconitase 2 Antibody | anti-ACO2 antibody
Goat anti-Aconitase 2 (aa541-555), Biotinylated Antibody
Gene Names
ACO2; ICRD; OCA8; OPA9; ACONM; HEL-S-284
Reactivity
Human, Mouse, Rat, PigExpected from sequence similarity: Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Aconitase 2; Goat anti-Aconitase 2 (aa541-555); Biotinylated Antibody; Goat Anti-Aconitase 2; Biotinlyated Antibody; ACO2; aconitase 2; ACONM; HEL-S-284; ICRD; OCA8; OPA9; mitochondrial; citrate hydro-lyase; epididymis secretory sperm binding protein Li 284; mitochondrial aconitase; anti-ACO2 antibody
Reactivity
Human, Mouse, Rat, Pig
Expected from sequence similarity: Dog, Cow
Expected from sequence similarity: Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
780
Applicable Applications for anti-ACO2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:16000.
WB: Approx 90kDa band observed in Human Heart lysates, in Human, Mouse and Rat Adipose lysates and in Mouse, Rat and Pig Skeletal Muscle lysates. Recommended concentration: 0.01-0.03ug/ml.
WB: Approx 90kDa band observed in Human Heart lysates, in Human, Mouse and Rat Adipose lysates and in Mouse, Rat and Pig Skeletal Muscle lysates. Recommended concentration: 0.01-0.03ug/ml.
Immunogen Sequence
Peptide with sequence CQDTYQHPPKDSSGQH., from the internal region of the protein sequence according to NP_001089.1.
Peptide Sequence + C
CQDTYQHPPKDSSGQH.
Epitope
Internal Region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
(Biotinylated (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
(Biotinylated (0.01ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85.4kDa
NCBI Official Full Name
aconitate hydratase, mitochondrial
NCBI Official Synonym Full Names
aconitase 2
NCBI Official Symbol
ACO2
NCBI Official Synonym Symbols
ICRD; OCA8; OPA9; ACONM; HEL-S-284
NCBI Protein Information
aconitate hydratase, mitochondrial
UniProt Protein Name
Aconitate hydratase, mitochondrial
Protein Family
UniProt Gene Name
ACO2
UniProt Synonym Gene Names
Aconitase
NCBI Description
The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq, Jul 2008]
Uniprot Description
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Research Articles on ACO2
Similar Products
Product Notes
The ACO2 aco2 (Catalog #AAA423742) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Aconitase 2 (aa541-555), Biotinylated Antibody reacts with Human, Mouse, Rat, Pig Expected from sequence similarity: Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Aconitase 2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Pep-ELISA: antibody detection limit dilution 1:16000. WB: Approx 90kDa band observed in Human Heart lysates, in Human, Mouse and Rat Adipose lysates and in Mouse, Rat and Pig Skeletal Muscle lysates. Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the ACO2 aco2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aconitase 2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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