Mouse Hormone-sensitive lipase ELISA Kit | Lipe elisa kit

Mouse Hormone-sensitive lipase ELISA Kit

Cat. Num. AAA762376

• Gene Names: Lipe; HSL; 4933403G17Rik
• Reactivity: Mouse
• Synonyms: Hormone-sensitive lipase; Mouse Hormone-sensitive lipase ELISA Kit; Lipe/HSL; Lipe elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lipe. No significant cross-reactivity or interference between Lipe and analogues was observed.
• Sequence Length: 759

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 0.938mIU/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipe were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipe were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Lipe elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Lipe antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Lipe antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Lipe amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Lipe can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 87239972
• NCBI GeneID: 16890
• NCBI Accession #: NP_001034596.1
• NCBI GenBank Nucleotide #: NM_001039507.2
• Molecular Weight: 88,027 Da
• NCBI Official Full Name: hormone-sensitive lipase isoform 2
• NCBI Official Synonym Full Names: lipase, hormone sensitive
• NCBI Official Symbol: Lipe
• NCBI Official Synonym Symbols: HSL; 4933403G17Rik
• NCBI Protein Information: hormone-sensitive lipase
• UniProt Protein Name: Hormone-sensitive lipase
• Protein Family: Hormone-sensitive lipase
• UniProt Gene Name: Lipe
• UniProt Synonym Gene Names: HSL
• UniProt Entry Name: LIPS_MOUSE

Uniprot Description

HSL: hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.

Protein type: Lipase; EC 3.1.1.79

Cellular Component: caveola; cytoplasm; cytosol; extracellular space; lipid particle; membrane; mitochondrion; nucleus; plasma membrane

Molecular Function: acylglycerol lipase activity; hormone-sensitive lipase activity; hydrolase activity; hydrolase activity, acting on ester bonds; lipase activity; protein binding; protein kinase binding; rRNA primary transcript binding; triacylglycerol lipase activity

Biological Process: cholesterol metabolic process; diacylglycerol catabolic process; lipid catabolic process; lipid metabolic process; long-chain fatty acid catabolic process; metabolic process; steroid metabolic process; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; triacylglycerol catabolic process