Typical Testing Data/Standard Curve (for reference only)
Mouse Acetylcholinesterase ELISA Kit | Ache elisa kit
Mouse Acetylcholinesterase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Membrane protein, GPI anchor; Lipid Metabolism - glycerophospholipid; EC 3.1.1.7
Cellular Component: axon; basal lamina; cell junction; cell soma; cell surface; cytoplasm; dendrite; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi apparatus; membrane; neuromuscular junction; neuron projection; nuclear envelope; perinuclear region of cytoplasm; plasma membrane; postsynaptic membrane; presynaptic membrane; rough endoplasmic reticulum; synapse
Molecular Function: 1-oxa-2-oxocycloheptane lactonase activity; 3,4-dihydrocoumarin hydrolase activity; acetylcholine binding; acetylcholinesterase activity; all-trans-retinyl-palmitate hydrolase activity; butyrolactone hydrolase activity; choline binding; cholinesterase activity; collagen binding; hydrolase activity; laminin binding; lipase activity; phospholipase activity; prenylcysteine methylesterase activity; protein binding; protein homodimerization activity; protein self-association; retinyl-palmitate esterase activity; serine hydrolase activity; sulfolactone hydrolase activity
Biological Process: acetylcholine catabolic process; acetylcholine metabolic process; cell adhesion; choline metabolic process; neurotransmitter catabolic process; neurotransmitter receptor biosynthetic process; positive regulation of axonogenesis; positive regulation of dendrite morphogenesis; protein tetramerization; receptor internalization; regulation of receptor recycling; retina development in camera-type eye; synaptogenesis
Research Articles on Ache
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Product Notes
The Mouse Ache ache (Catalog #AAA2883100) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2883100 ELISA Kit recognizes Mouse Ache. It is sometimes possible for the material contained within the vial of "Acetylcholinesterase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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