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Typical Testing Data/Standard Curve (for reference only)

Human acetylcholinesterase (Yt blood group) ELISA Kit | ACHE elisa kit

Human Acetylcholinesterase, AChE ELISA Kit

Gene Names
ACHE; YT; ACEE; ARACHE; N-ACHE
Reactivity
Human
Synonyms
acetylcholinesterase (Yt blood group); Human Acetylcholinesterase; AChE ELISA Kit; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase; ACHE elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ACHE. No significant cross-reactivity or interference between Human ACHE and analogues was observed.
Sequence Length
614
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.94 mU/ml-60 mU/ml
Sensitivity
0.24 mU/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Neurobiology
Protein Biological Process 3
Neurotransmitter degradation
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ACHE elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AChE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AChE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AChE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AChE bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
43
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,796 Da
NCBI Official Full Name
acetylcholinesterase isoform E4-E6
NCBI Official Synonym Full Names
acetylcholinesterase (Yt blood group)
NCBI Official Symbol
ACHE
NCBI Official Synonym Symbols
YT; ACEE; ARACHE; N-ACHE
NCBI Protein Information
acetylcholinesterase; Yt blood group; apoptosis-related acetylcholinesterase
UniProt Protein Name
Acetylcholinesterase
UniProt Gene Name
ACHE
UniProt Synonym Gene Names
AChE
UniProt Entry Name
ACES_HUMAN

NCBI Description

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.4 PublicationsManual assertion based on experiment in:Ref.11

Catalytic activity: Acetylcholine + H2O = choline + acetate.

Research Articles on ACHE

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Product Notes

The Human ACHE ache (Catalog #AAA704993) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA704993 ELISA Kit recognizes Human ACHE. It is sometimes possible for the material contained within the vial of "acetylcholinesterase (Yt blood group), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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