Mouse Acetylcholinesterase ELISA Kit | Ache elisa kit

Mouse Acetylcholinesterase ELISA Kit

Cat. Num. AAA763279

• Gene Names: ACHE; YT; ACEE; ARACHE; N-ACHE
• Reactivity: Mouse
• Synonyms: Acetylcholinesterase; Mouse Acetylcholinesterase ELISA Kit; Ache (Acetylcholinesterase)/ACEE/ARACHE/N-ACHE/YT/Acetylcholinesterase YT blood group/Apoptosis related acetylcholinesterase; Ache elisa kit

• Reactivity: Mouse
• Sequence Length: 72

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Ache elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Ache antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Ache antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Ache amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Ache can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 545225
• NCBI GeneID: 43
• Molecular Weight: 58,352 Da
• NCBI Official Full Name: acetylcholinesterase, partial
• NCBI Official Synonym Full Names: acetylcholinesterase (Yt blood group)
• NCBI Official Symbol: ACHE
• NCBI Official Synonym Symbols: YT; ACEE; ARACHE; N-ACHE
• NCBI Protein Information: acetylcholinesterase
• UniProt Protein Name: Acetylcholinesterase
• Protein Family: Acetylcholinesterase
• UniProt Gene Name: ACHE
• UniProt Synonym Gene Names: AChE
• UniProt Entry Name: ACES_HUMAN

NCBI Description

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

Uniprot Description

ACHE: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. Belongs to the type-B carboxylesterase/lipase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Lipid Metabolism - glycerophospholipid; EC 3.1.1.7; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: basal lamina; cell junction; cell surface; extracellular region; extracellular space; Golgi apparatus; membrane; neuromuscular junction; nucleus; perinuclear region of cytoplasm; plasma membrane; synapse

Molecular Function: acetylcholine binding; acetylcholinesterase activity; beta-amyloid binding; cholinesterase activity; collagen binding; hydrolase activity; laminin binding; protein binding; protein homodimerization activity; protein self-association; serine hydrolase activity

Biological Process: acetylcholine catabolic process; acetylcholine catabolic process in synaptic cleft; amyloid precursor protein metabolic process; cell adhesion; cell proliferation; DNA replication; glycerophospholipid biosynthetic process; muscle development; negative regulation of synaptic transmission, cholinergic; nervous system development; neurotransmitter biosynthetic process; neurotransmitter receptor biosynthetic process; osteoblast development; phosphatidylcholine biosynthetic process; phospholipid metabolic process; positive regulation of protein secretion; protein tetramerization; receptor internalization; regulation of receptor recycling; response to wounding; retina development in camera-type eye; synaptic transmission; synaptogenesis

Disease: Yt Blood Group Antigen