Typical Testing Data/Standard Curve (for reference only)
Rat chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) ELISA Kit | CXCL1 elisa kit
Rat growth-regulated oncogene alpha/melanoma growth stimulating activity, GROalpha /MGSA ELISA Kit
Gene Names
Cxcl1; Gro1; CINC-1
Reactivity
Rat
Synonyms
chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity; alpha); Rat growth-regulated oncogene alpha/melanoma growth stimulating activity; GROalpha /MGSA ELISA Kit; GROalpha /MGSA Elisa Kit; FSP; GRO1; GROa; MGSA; MGSA-a; NAP-3; SCYB1; GRO1 oncogene (melanoma growth stimulating activity; alpha) ; GRO1 oncogene (melanoma growth-stimulating activity) ; MGSA alpha; chemokine (C-X-C motif) ligan; CXCL1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat GROalpha /MGSA. No significant cross-reactivity or interference between rat GROalpha /MGSA and analogues was observed.
Sequence Length
96
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
125 pg/ml -8000 pg/ml.
Sensitivity
The minimum detectable dose of rat GROalpha /MGSA is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CXCL1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GROalpha /MGSA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GROalpha /MGSA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GROalpha /MGSA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GROalpha /MGSA bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,249 Da
NCBI Official Full Name
growth-regulated alpha protein
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 1
NCBI Official Symbol
Cxcl1
NCBI Official Synonym Symbols
Gro1; CINC-1
NCBI Protein Information
growth-regulated alpha protein; C-X-C motif chemokine 1; cytokine-induced neutrophil chemoattractant 1; platelet-derived growth factor-inducible protein KC; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
UniProt Protein Name
Growth-regulated alpha protein
Protein Family
UniProt Gene Name
Cxcl1
UniProt Synonym Gene Names
Cinc1; Gro; Scyb1; CINC-1
UniProt Entry Name
GROA_RAT
NCBI Description
acts as a neutrophil chemoattractant; may play a role in acute phase inflammatory response [RGD, Feb 2006]
Uniprot Description
Function: Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.
Research Articles on CXCL1
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Product Notes
The Rat CXCL1 cxcl1 (Catalog #AAA704641) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA704641 ELISA Kit recognizes Rat CXCL1. It is sometimes possible for the material contained within the vial of "chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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