ACAA2 recombinant protein

Recombinant Human ACAA2 protein

Cat. Num. AAA1561977

• Gene Names: ACAA2; DSAEC
• Purity: >90% as determined by SDS-PAGE
• Synonyms: ACAA2; Recombinant Human ACAA2 protein; 3-ketoacyl-CoA thiolase; mitochondrial; Acetyl-CoA acetyltransferase; Acetyl-CoA acyltransferase; Acyl-CoA hydrolase; Beta-ketothiolase; Mitochondrial 3-oxoacyl-CoA thiolase; T1; ACAA2 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human ACAA2 (Arg90-Ala397) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for ACAA2 recombinant protein

In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies. Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation. Abolishes BNIP3-mediated apoptosis and mitochondrial damage.

Product Categories/Family for ACAA2 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 167614485
• NCBI GeneID: 10449
• NCBI Accession #: NP_006102.2
• NCBI GenBank Nucleotide #: NM_006111.2
• UniProt Accession #: P42765; Q9BUT6
• Molecular Weight: 41,924 Da
• NCBI Official Full Name: 3-ketoacyl-CoA thiolase, mitochondrial
• NCBI Official Synonym Full Names: acetyl-CoA acyltransferase 2
• NCBI Official Symbol: ACAA2
• NCBI Official Synonym Symbols: DSAEC
• NCBI Protein Information: 3-ketoacyl-CoA thiolase, mitochondrial; T1; acetyl-Coenzyme A acyltransferase 2; beta ketothiolase; beta-ketothiolase; mitochondrial 3-oxoacyl-CoA thiolase; mitochondrial 3-oxoacyl-Coenzyme A thiolase
• UniProt Protein Name: 3-ketoacyl-CoA thiolase, mitochondrial
• UniProt Gene Name: ACAA2
• UniProt Entry Name: THIM_HUMAN

NCBI Description

The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. [provided by RefSeq, Jul 2008]

Uniprot Description

ACAA2: Abolishes BNIP3-mediated apoptosis and mitochondrial damage. Belongs to the thiolase family.

Protein type: Lipid Metabolism - fatty acid; EC 2.3.1.16; Lipid Metabolism - fatty acid elongation in mitochondria; Transferase; Amino Acid Metabolism - valine, leucine and isoleucine degradation

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: protein binding; acetyl-CoA C-acyltransferase activity

Biological Process: fatty acid metabolic process; cholesterol biosynthetic process