ATP Binding Cassette Transporter G1 (ABCG1) Recombinant Protein | ABCG1 recombinant protein

Recombinant ATP Binding Cassette Transporter G1 (ABCG1)

Cat. Num. AAA2011718

• Gene Names: ABCG1; ABC8; WHITE1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: ATP Binding Cassette Transporter G1 (ABCG1); Recombinant ATP Binding Cassette Transporter G1 (ABCG1); ABCG1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-FR DLSYSVPEGP WWRKKGYKTL LKGISGKFNS GELVAIMGPS GAGKSTLMNI LAGYRETGMK GAVLINGLPR DLRCFRKVSC YIMQDDMLLP HLTVQEAMMV SAHLKLQEKD EGRREMVKEI LTALGLLSCA NTRTGSLSGG QRKRLAIALE LVNNPPVMFF DEPTSGLDSA SCFQVVSLMK GLAQGGRSII CTIHQPSAKL FELFDQLYVL SQGQCVYRGK VCNLVPYLRD LG
• Sequence Length: 678

• Applicable Applications for ABCG1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Phe79~Gly312 (Accession # P45844) with N-terminal His-Tag
• Subcellular Location: Mitochondrion. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9.2
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 46592915
• NCBI GeneID: 9619
• NCBI Accession #: NP_004906.3
• NCBI GenBank Nucleotide #: NM_004915.3
• UniProt Accession #: P45844; Q86SU8; Q96L76; Q9BXK6; Q9BXK7; Q9BXK8; Q9BXK9; Q9BXL0; Q9BXL1; Q9BXL2; Q9BXL3; Q9BXL4
• Molecular Weight: 27.3kDa
• NCBI Official Full Name: ATP-binding cassette sub-family G member 1 isoform 4
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family G (WHITE), member 1
• NCBI Official Symbol: ABCG1
• NCBI Official Synonym Symbols: ABC8; WHITE1
• NCBI Protein Information: ATP-binding cassette sub-family G member 1; ABC transporter 8; homolog of Drosophila white; ATP-binding cassette transporter 8; ATP-binding cassette transporter member 1 of subfamily G; white protein homolog (ATP-binding cassette transporter 8)
• UniProt Protein Name: ATP-binding cassette sub-family G member 1
• Protein Family: ABC transporter G family
• UniProt Gene Name: ABCG1
• UniProt Synonym Gene Names: ABC8; WHT1
• UniProt Entry Name: ABCG1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ABCG1: Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ABC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; recycling endosome; mitochondrion; intracellular membrane-bound organelle; integral to plasma membrane; plasma membrane; endosome; external side of plasma membrane

Molecular Function: protein dimerization activity; protein binding; protein homodimerization activity; phospholipid transporter activity; protein heterodimerization activity; cholesterol transporter activity; toxin transporter activity; cholesterol binding; phospholipid binding; sterol-transporting ATPase activity; ADP binding; ATP binding

Biological Process: cholesterol metabolic process; detection of hormone stimulus; response to high density lipoprotein stimulus; positive regulation of cholesterol biosynthetic process; cholesterol efflux; lipoprotein metabolic process; intracellular cholesterol transport; response to lipid; response to organic substance; cholesterol homeostasis; amyloid precursor protein catabolic process; reverse cholesterol transport; phospholipid efflux; regulation of transcription, DNA-dependent; transmembrane transport; phospholipid homeostasis