alanyl-tRNA synthetase Recombinant Protein | AARS recombinant protein

alanyl-tRNA synthetase Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122713

• Gene Names: Aars; sti; C76919; AI316495
• Purity: >88% as determined by SDS-PAGE
• Synonyms: alanyl-tRNA synthetase; alanyl-tRNA synthetase Protein; Mouse; Recombinant (His Tag); Mouse AARS/alanyl-tRNA synthetase Protein (His Tag); AI316495 Protein; C76919 Protein; sti Protein; AARS recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >88% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn968

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse AARS (Q8BGQ7)(Met 1-Asn 968) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for AARS recombinant protein

Background: Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections.

References

Wakasugi K, et al. (1999) Two Distinct Cytokines Released from a Human Aminoacyl-tRNA Synthetase. Science. 284 (5411): 147-51.Sokabe M, et al. (2009) The structure of alanyl-tRNA synthetase with editing domain. Proc Natl Acad Sci. 106 (27): 11028-33.Skupinska M, et al. (2009) AARS--the etiological factor and the attractive target of many disorders. Postepy Biochem. 55 (4): 373-84.

NCBI and Uniprot Product Information

• NCBI GI #: 34610207
• NCBI GeneID: 234734
• NCBI Accession #: NP_666329.2
• NCBI GenBank Nucleotide #: NM_146217.4
• UniProt Accession #: Q8BGQ7; Q8BXR0
• Molecular Weight: 106,909 Da
• NCBI Official Full Name: alanine--tRNA ligase, cytoplasmic
• NCBI Official Synonym Full Names: alanyl-tRNA synthetase
• NCBI Official Symbol: Aars
• NCBI Official Synonym Symbols: sti; C76919; AI316495
• NCBI Protein Information: alanine--tRNA ligase, cytoplasmic; alaRS; alanyl-tRNA synthetase, cytoplasmic
• UniProt Protein Name: Alanine--tRNA ligase, cytoplasmic
• UniProt Gene Name: Aars
• UniProt Synonym Gene Names: AlaRS
• UniProt Entry Name: SYAC_MOUSE