Mouse a1-microglobulin ELISA Kit | A1-MG elisa kit

Mouse a1-microglobulin ELISA Kit

Cat. Num. AAA731707

• Gene Names: AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
• Reactivity: Mouse
• Synonyms: a1-microglobulin; Mouse a1-microglobulin ELISA Kit; A1-MG elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This alpha1-MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse alpha1-MG. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for A1-MG elisa kit

Principle of the Assay: alpha1-MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-alpha1-MG antibody and an alpha1-MG-HRP conjugate. The assay sample and buffer are incubated together with alpha1-MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the alpha1-MG concentration since alpha1-MG from samples and alpha1-MG-HRP conjugate compete for the anti-alpha1-MG antibody binding site. Since the number of sites is limited, as more sites are occupied by alpha1-MG from the sample, fewer sites are left to bind alpha1-MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The alpha1-MG concentration in each sample is interpolated from this standard curve.

Product Categories/Family for A1-MG elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 579676
• NCBI GeneID: 259
• UniProt Accession #: P02760; P00977; P02759; P78491; Q2TU33; Q5TBD7; Q9UC58; Q9UDI8
• Molecular Weight: 38,999 Da
• NCBI Official Full Name: alpha-1-microglobulin, partial
• NCBI Official Synonym Full Names: alpha-1-microglobulin/bikunin precursor
• NCBI Official Symbol: AMBP
• NCBI Official Synonym Symbols: A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
• NCBI Protein Information: protein AMBP; bikunin; uristatin; protein HC; trypstatin; uronic-acid-rich protein; growth-inhibiting protein 19; inter-alpha-trypsin inhibitor light chain; complex-forming glycoprotein heterogeneous in charge
• UniProt Protein Name: Protein AMBP
• UniProt Gene Name: AMBP
• UniProt Synonym Gene Names: HCP; ITIL; Protein HC; ITI-LC
• UniProt Entry Name: AMBP_HUMAN

NCBI Description

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]

Uniprot Description

AMBP: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q32-q33

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; extracellular region; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; calcium oxalate binding; IgA binding; heme binding; calcium channel inhibitor activity

Biological Process: receptor-mediated endocytosis; negative regulation of JNK cascade; viral reproduction; heme catabolic process; negative regulation of immune response; protein catabolic process; female pregnancy; cell adhesion; protein-chromophore linkage