Growth Differentiation Factor 3 Active Protein

Growth Differentiation Factor 3, Recombinant, Mouse (GDF3)

Cat. Num. AAA650309

• Purity: Highly Purified; >90% as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Growth Differentiation Factor 3; Recombinant; Mouse (GDF3); Growth Differentiation Factor 3 active protein

• Host: A DNA sequence encoding mouse GDF-3 (Ala 253-Gly 366)
• Purity/Purification: Highly Purified; >90% as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder from 30% Acetonitrile, 0.1% TFA.

• Activity: Measured by its ability to bind with Activan RIB/Fc Chimera in the presence of recombinant mouse Cripto in functional ELISA. Immonilized recombinant mouse GDF3 at 1ug/ml (100ul/well) can bind recombinant human Activin RIB/Fc Chimera with an apparent KD <100nM.
• Accession Number: Q07104
• Endotoxin: <0.01EU/ug (LAL)
• Reconstitution: Reconstitute with sterile 4mM HCl to a concentration of 0.1mg/ml
• Molecular Mass: Based on N-terminal sequencing, the refolded mature mouse GDF-3 protein begins with Ala 253. The recombinant monomeric mouse GDF-3 has a calculated molecular mass of 13.1kD.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 4mM HCl. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Stable for 3 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Growth Differentiation Factor 3 active protein

GDF3 (previously called Vgr-2) is a TGF-beta superfamily member belonging to the growth/differentiation factor family. GDF3 is expressed in undifferentiated embryonic stem (ES) cells, adipose tissue and the brain. In ES cells, it maintains pluripotency and influences early cell fate decisions. For example, frog embryos injected with GDF3 develop a secondary dorsal axis and deletion of mouse GDF3 can produce defects in the anterior visceral endoderm of the pre-gastrulation embryo. In adipocytes, GDF3 is induced by a high fat diet and promotes adipogenesis. GDF3 has been reported to oppose BMP's functions and to have a nodal-like activity in the early development. The 366aa mouse GDF3 contains a 22aa signal sequence, a 230aa propeptide and a 114aa mature protein that contains one potential N-glycosylation site. Most of GDF3 is present as the prepro form, while mature GDF3 is presumably the secreted, active form. The mature protein contains the cysteine-knot structure that is conserved throughout family members. Since it lacks the fourth cysteine, which is responsible for the formation of inter-molecular disulfide bond, GDF3 may exist as a non-covalent homodimer. Mature mouse GDF3 shares 90%, 83% and 83% aa identity with rat, human and canine GDF3, respectively. Among family members, mature GDF3 is most similar to mouse BMP-6 (45% aa identity) and Xenopus VG-1 (52% aa identity).

Product Categories/Family for Growth Differentiation Factor 3 active protein

Growth Factors; Cytokines; Growth Factors-GDF

NCBI and Uniprot Product Information

• NCBI GI #: 9652072
• Molecular Weight: Based on N-terminal sequencing, the refolded mature mouse GDF-3 protein begins with Ala 253. The recombinant monomeric mouse GDF-3 has a calculated molecular mass of 13.1 kD.
• NCBI Official Full Name: growth differentiation factor 3

Uniprot Description

GDF3: Defects in GDF3 are the cause of Klippel-Feil syndrome type 3 (KFS3); also called Klippel-Feil syndrome autosomal dominant 3. KFS3 is a skeletal disorder characterized by congenital fusion of cervical vertebrae. It is due to a failure in the normal segmentation of vertebrae during the early weeks of fetal development. The clinical triad consists of short neck, low posterior hairline, and limited neck movement. Defects in GDF3 are the cause of microphthalmia isolated with coloboma type 6 (MCOPCB6); also called isolated colobomatous microphthalmia 6. MCOPCB6 is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Defects in GDF3 are a cause of microphthalmia isolated type 7 (MCOP7). MCOP7 is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: extracellular space; cytoplasm

Molecular Function: growth factor activity; cytokine activity; protein kinase binding; transforming growth factor beta receptor binding

Biological Process: response to dietary excess; in utero embryonic development; somite rostral/caudal axis specification; notochord development; formation of anatomical boundary; negative regulation of BMP signaling pathway; regulation of apoptosis; eye development; regulation of MAPKKK cascade; regulation of cell fate commitment; negative regulation of epidermal cell differentiation; mesoderm development; negative regulation of myoblast differentiation; cell development; skeletal development; endoderm development; growth

Disease: Microphthalmia, Isolated 7; Klippel-feil Syndrome 3, Autosomal Dominant; Microphthalmia, Isolated, With Coloboma 6