GM-CSF active protein

Mouse GM-CSF Protein

Cat. Num. AAA9718726

• Gene Names: Csf2; CSF; Csfgm; GMCSF; Gm-CSf; MGI-IGM
• Purity: >95% as determined by SDS-PAGE
• Synonyms: GM-CSF; Mouse GM-CSF Protein; Recombinant Mouse GM-CSF Protein (Granulocyte-macrophage colony-stimulating factor) expressed in Human Cells; Csfgm; Gm-CSf; GMCSF; MGI-IGM; Granulocyte-macrophage colony-stimulating factor; CSF2; GM-CSF active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4.
• Sequence: Amino acid sequence derived from mouse CSF2 (NP_034099.2) (Met1-Lys141) was expressed.
• Sequence Length: 141

• Species: Mouse
• Endotoxin: The Endotoxin level is less than 1.0 EU per mug of the protein as determined by the LAL method; Protein Length
• Activity: Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 0.012-0.047 ng/mL. The ED50 for this effect is typically 0.012-0.047 ng/mL.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse GM-CSF protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Mouse GM-CSF protein.)

Related Product Information for GM-CSF active protein

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is one of an array of cytokines with pivotal roles in embryo implantation and subsequent development. Several cell lineages in the reproductive tract and gestational tissues synthesise GM-CSF under direction by ovarian steroid hormones and signalling agents originating in male seminal fluid and the conceptus. The pre-implantation embryo, invading placental trophoblast cells and the abundant populations of leukocytes controlling maternal immune tolerance are all subject to GM-CSF regulation. GM-CSF stimulates the differentiation of hematopoietic progenitors to monocytes and neutrophils, and reduces the risk for febrile neutropenia in cancer patients. GM-CSF also has been shown to induce the differentiation of myeloid dendritic cells (DCs) that promote the development of T-helper type 1 (cellular) immune responses in cognate T cells. The active form of the protein is found extracellularly as a homodimer, and the encoding gene is localized to a related gene cluster at chromosome region 5q31 which is known to be associated with 5q-syndrome and acute myelogenous leukemia. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has been utilized in the clinical management of multiple disease processes. Most recently, GM-CSF has been incorporated into the treatment of malignancies as a sole therapy, as well as a vaccine adjuvant. While the benefits of GM-CSF in this arena have been promising, recent reports have suggested the potential for GM-CSF to induce immune suppression and, thus, negatively impact outcomes in the management of cancer patients. GM-CSF deficiency in pregnancy adversely impacts fetal and placental development, as well as progeny viability and growth after birth, highlighting this cytokine as a central maternal determinant of pregnancy outcome with clinical relevance in human fertility.

NCBI and Uniprot Product Information

• NCBI GI #: 110625658
• NCBI GeneID: 12981
• NCBI Accession #: NP_034099.2
• NCBI GenBank Nucleotide #: NP_034099.2
• UniProt Accession #: P01587
• Molecular Weight: 14.1 kDa
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: Csf2
• NCBI Official Synonym Symbols: CSF; Csfgm; GMCSF; Gm-CSf; MGI-IGM
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor