Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pogo transposable element with KRAB domain (POGK) Standard Curve.)
Mouse Pogo Transposable Element With KRAB Domain (POGK) ELISA Kit | POGK elisa kit
Mouse Pogo Transposable Element With KRAB Domain (POGK) ELISA Kit
Gene Names
Pogk; BASS2; mKIAA1513; 9130401E23Rik
Reactivity
Mouse
Synonyms
Pogo Transposable Element With KRAB Domain (POGK); Mouse Pogo Transposable Element With KRAB Domain (POGK) ELISA Kit; POGK; BASS2; KIAA1513; KIAA15131; LST003; OTTHUMP00000032216; POGK elisa kit
Reactivity
Mouse
Specificity
Mouse Pogo transposable element with KRAB domain (POGK) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POGK. No significant cross-reactivity or interference between Mouse POGK and analogues was observed.
Sequence Length
626
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pogo transposable element with KRAB domain (POGK) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pogo transposable element with KRAB domain (POGK) Standard Curve.)
Related Product Information for POGK elisa kit
Description: This Mouse Pogo transposable element with KRAB domain (POGK) ELISA Kit employs a two-site sandwich ELISA to quantitate POGK in samples. An antibody specific for POGK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POGK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POGK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POGK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The exact function of the protein encoded by POGK is not known. However, this gene product contains a KRAB domain (which is involved in protein-protein interactions) at the N-terminus, and a transposase domain at the C-terminus, suggesting that it may belong to the family of DNA-mediated transposons in human. The Krueppel-associated box (KRAB) is a domain of around 75 amino acids that is found in the N-terminal part of about one third of eukaryotic Krueppel-type C2H2 zinc finger proteins (ZFPs). It is enriched in charged amino acids and can be divided into subregions A and B, which are predicted to fold into two amphipathic alpha-helices. The KRAB A and B boxes can be separated by variable spacer segments and many KRAB proteins contain only the A box.
Background: The exact function of the protein encoded by POGK is not known. However, this gene product contains a KRAB domain (which is involved in protein-protein interactions) at the N-terminus, and a transposase domain at the C-terminus, suggesting that it may belong to the family of DNA-mediated transposons in human. The Krueppel-associated box (KRAB) is a domain of around 75 amino acids that is found in the N-terminal part of about one third of eukaryotic Krueppel-type C2H2 zinc finger proteins (ZFPs). It is enriched in charged amino acids and can be divided into subregions A and B, which are predicted to fold into two amphipathic alpha-helices. The KRAB A and B boxes can be separated by variable spacer segments and many KRAB proteins contain only the A box.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pogo transposable element with KRAB domain isoform 2
NCBI Official Synonym Full Names
pogo transposable element with KRAB domain
NCBI Official Symbol
Pogk
NCBI Official Synonym Symbols
BASS2; mKIAA1513; 9130401E23Rik
NCBI Protein Information
pogo transposable element with KRAB domain
UniProt Protein Name
Pogo transposable element with KRAB domain
Protein Family
UniProt Gene Name
Pogk
UniProt Synonym Gene Names
Kiaa1513
UniProt Entry Name
POGK_MOUSE
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Product Notes
The Mouse POGK pogk (Catalog #AAA9718056) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718056 ELISA Kit recognizes Mouse POGK. It is sometimes possible for the material contained within the vial of "Pogo Transposable Element With KRAB Domain (POGK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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