Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cell surface glycoprotein MUC18 (MCAM) Standard Curve.)
Mouse Cell Surface Glycoprotein MUC18 (MCAM) ELISA Kit | MCAM elisa kit
Mouse Cell Surface Glycoprotein MUC18 (MCAM) ELISA Kit
Gene Names
Mcam; CD146; CD149; Muc18; s-endo; AV025631; 1-gicerin; s-gicerin
Reactivity
Mouse
Synonyms
Cell Surface Glycoprotein MUC18 (MCAM); Mouse Cell Surface Glycoprotein MUC18 (MCAM) ELISA Kit; MCAM; CD146; MUC18; Gicerin; melanoma adhesion molecule; MCAM elisa kit
Reactivity
Mouse
Specificity
Mouse Cell surface glycoprotein MUC18 (MCAM) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MCAM. No significant cross-reactivity or interference between Mouse MCAM and analogues was observed.
Sequence Length
648
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cell surface glycoprotein MUC18 (MCAM) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Cell surface glycoprotein MUC18 (MCAM) Standard Curve.)
Related Product Information for MCAM elisa kit
Description: This Mouse Cell surface glycoprotein MUC18 (MCAM) ELISA Kit employs a two-site sandwich ELISA to quantitate MCAM in samples. An antibody specific for MCAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCAM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: CD146 (cluster of differentiation 146) is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene. Its function is still poorly understood, but evidence points to it being part of the endothelial junction associated with the actin cytoskeleton. A member of the Immunoglobulin superfamily, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle.
Background: CD146 (cluster of differentiation 146) is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene. Its function is still poorly understood, but evidence points to it being part of the endothelial junction associated with the actin cytoskeleton. A member of the Immunoglobulin superfamily, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cell surface glycoprotein MUC18 isoform 1
NCBI Official Synonym Full Names
melanoma cell adhesion molecule
NCBI Official Symbol
Mcam
NCBI Official Synonym Symbols
CD146; CD149; Muc18; s-endo; AV025631; 1-gicerin; s-gicerin
NCBI Protein Information
cell surface glycoprotein MUC18
UniProt Protein Name
Cell surface glycoprotein MUC18
Protein Family
UniProt Gene Name
Mcam
UniProt Synonym Gene Names
Muc18
Uniprot Description
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration ().
Research Articles on MCAM
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Product Notes
The Mouse MCAM mcam (Catalog #AAA9717707) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717707 ELISA Kit recognizes Mouse MCAM. It is sometimes possible for the material contained within the vial of "Cell Surface Glycoprotein MUC18 (MCAM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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