Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein kinase C alpha type (PRKCA) Standard Curve.)
Mouse Protein Kinase C alpha Type (PRKCA) ELISA Kit | PRKCA elisa kit
Mouse Protein Kinase C alpha Type (PRKCA) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein kinase C alpha type (PRKCA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein kinase C alpha type (PRKCA) Standard Curve.)
Background: PRKCA, refers to both a human gene and the protein that is encoded by it. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control.
NCBI and Uniprot Product Information
Uniprot Description
PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators.
Protein type: Kinase, protein; Protein kinase, AGC; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; AGC group; PKC family; Alpha subfamily
Cellular Component: apical part of cell; axon; cell soma; cytoplasm; cytosol; dendrite; endoplasmic reticulum; lipid raft; membrane; mitochondrion; neuron projection; nucleus; perinuclear region of cytoplasm; photoreceptor outer segment; plasma membrane; protein complex
Molecular Function: ATP binding; calcium-dependent protein kinase C activity; enzyme binding; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity; transferase activity; zinc ion binding
Biological Process: angiogenesis; apoptosis; cell adhesion; cellular calcium ion homeostasis; central nervous system neuron axonogenesis; chondrocyte differentiation; inactivation of MAPK activity; induction of positive chemotaxis; learning and/or memory; negative regulation of cell proliferation; negative regulation of glucose import; negative regulation of heart contraction; negative regulation of insulin receptor signaling pathway; negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase activity; negative regulation of translation; neutrophil chemotaxis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of cell adhesion; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of exocytosis; positive regulation of inflammatory response; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of macrophage differentiation; positive regulation of mitotic cell cycle; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of synaptogenesis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of muscle contraction; regulation of peptidyl-tyrosine phosphorylation; regulation of receptor-mediated endocytosis; regulation of the force of heart contraction; response to estradiol stimulus; response to ethanol; response to reactive oxygen species
Research Articles on PRKCA
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Product Notes
The Mouse PRKCA prkca (Catalog #AAA9717276) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717276 ELISA Kit recognizes Mouse PRKCA. It is sometimes possible for the material contained within the vial of "Protein Kinase C alpha Type (PRKCA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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