Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transforming growth factor-beta-induced protein ig-h3 (TGFBI) Standard Curve.)
Mouse Transforming Growth Factor-beta-Induced Protein ig-h3 (TGFBI) ELISA Kit | TGFBI elisa kit
Mouse Transforming Growth Factor-beta-Induced Protein ig-h3 (TGFBI) ELISA Kit
Gene Names
Tgfbi; 68kDa; big-h3; Beta-ig; AI181842; AI747162
Reactivity
Mouse
Synonyms
Transforming Growth Factor-beta-Induced Protein ig-h3 (TGFBI); Mouse Transforming Growth Factor-beta-Induced Protein ig-h3 (TGFBI) ELISA Kit; TGFBI; BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; LCD1; RGD-containing collagen-associated protein; kerato-epithelin; TGFBI elisa kit
Reactivity
Mouse
Specificity
Mouse Transforming growth factor-beta-induced protein ig-h3 (TGFBI) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TGFBI. No significant cross-reactivity or interference between Mouse TGFBI and analogues was observed.
Sequence Length
683
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transforming growth factor-beta-induced protein ig-h3 (TGFBI) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Transforming growth factor-beta-induced protein ig-h3 (TGFBI) Standard Curve.)
Related Product Information for TGFBI elisa kit
Description: This Mouse Transforming growth factor-beta-induced protein ig-h3 (TGFBI) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFBI in samples. An antibody specific for TGFBI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGFBI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFBI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFBI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TGF-beta is capable of producing a variety of effects and virtually all cell types respond to this factor in some way. The inappropriate presence of active TGF-beta1 has been implicated in a variety of pathological conditions Because of the necessity for regulating its activity tightly, TGF-beta is secreted by cells in the form of an inactive complex. This complex consists of TGF-beta1 associated non-covalently with a protein designated the latency associated peptide (LAP). TGF-beta1 and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. LAP and TGF-beta1 each consist of a disulfide-linked homodimer and the association of these two components renders TGF-beta1 inactive and inaccessible to anti-TGF-beta antibodies.
Background: TGF-beta is capable of producing a variety of effects and virtually all cell types respond to this factor in some way. The inappropriate presence of active TGF-beta1 has been implicated in a variety of pathological conditions Because of the necessity for regulating its activity tightly, TGF-beta is secreted by cells in the form of an inactive complex. This complex consists of TGF-beta1 associated non-covalently with a protein designated the latency associated peptide (LAP). TGF-beta1 and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. LAP and TGF-beta1 each consist of a disulfide-linked homodimer and the association of these two components renders TGF-beta1 inactive and inaccessible to anti-TGF-beta antibodies.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transforming growth factor-beta-induced protein ig-h3
NCBI Official Synonym Full Names
transforming growth factor, beta induced
NCBI Official Symbol
Tgfbi
NCBI Official Synonym Symbols
68kDa; big-h3; Beta-ig; AI181842; AI747162
NCBI Protein Information
transforming growth factor-beta-induced protein ig-h3
UniProt Protein Name
Transforming growth factor-beta-induced protein ig-h3
Protein Family
UniProt Gene Name
Tgfbi
UniProt Synonym Gene Names
Beta ig-h3
UniProt Entry Name
BGH3_MOUSE
Uniprot Description
betaIG-H3: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. By TGFB1. Highly expressed in the corneal epithelium.
Protein type: Secreted, signal peptide; Extracellular matrix; Secreted; Cell adhesion
Cellular Component: basement membrane; extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix; trans-Golgi network
Molecular Function: cell adhesion molecule binding; collagen binding; extracellular matrix binding; integrin binding
Biological Process: cell adhesion; cell proliferation; extracellular matrix organization and biogenesis
Research Articles on TGFBI
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Product Notes
The Mouse TGFBI tgfbi (Catalog #AAA9716979) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716979 ELISA Kit recognizes Mouse TGFBI. It is sometimes possible for the material contained within the vial of "Transforming Growth Factor-beta-Induced Protein ig-h3 (TGFBI), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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