Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cathepsin H (CTSH) Standard Curve.)
Human Cathepsin H (CTSH) ELISA Kit | CTSH elisa kit
Human Cathepsin H (CTSH) ELISA Kit
Reactivity
Human
Synonyms
Cathepsin H (CTSH); Human Cathepsin H (CTSH) ELISA Kit; CTSH; ACC-4; ACC-5; CPSB; DKFZp686B24257; MGC1519; minichain; N-benzoylarginine-beta-naphthylamide hydrolase; aleurain; cathepsin B3; cathepsin BA; CTSH elisa kit
Reactivity
Human
Specificity
Human Cathepsin H (CTSH) ELISA Kit has high sensitivity and excellent specificity for detection of Human CTSH. No significant cross-reactivity or interference between Human CTSH and analogues was observed.
Sequence Length
335
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cathepsin H (CTSH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cathepsin H (CTSH) Standard Curve.)
Related Product Information for CTSH elisa kit
Description: This Human Cathepsin H (CTSH) ELISA Kit employs a two-site sandwich ELISA to quantitate CTSH in samples. An antibody specific for CTSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTSH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Cathepsin H is is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor.The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors.Two transcript variants encoding different isoforms have been found for this gene.One form of Batten disease, representing perhaps about one-fourth of all cases, has been found to show deficiency of cathepsin H. The Batten disease phenotype maps to chromosome 16, however.
Background: Cathepsin H is is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor.The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors.Two transcript variants encoding different isoforms have been found for this gene.One form of Batten disease, representing perhaps about one-fourth of all cases, has been found to show deficiency of cathepsin H. The Batten disease phenotype maps to chromosome 16, however.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pro-cathepsin H
NCBI Official Synonym Full Names
cathepsin H
NCBI Official Symbol
CTSH
NCBI Protein Information
pro-cathepsin H
UniProt Protein Name
Pro-cathepsin H
Protein Family
UniProt Gene Name
CTSH
UniProt Entry Name
CATH_BOVIN
Uniprot Description
Function: Important for the overall degradation of proteins in lysosomes
Catalytic activity: Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase.
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Product Notes
The Human CTSH ctsh (Catalog #AAA9716341) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716341 ELISA Kit recognizes Human CTSH. It is sometimes possible for the material contained within the vial of "Cathepsin H (CTSH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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