Human CTSH (Cathepsin H) ELISA Kit | CTSH elisa kit

Human CTSH (Cathepsin H) ELISA Kit

Cat. Num. AAA7612312

• Gene Names: CTSH; CPSB; ACC-4; ACC-5; minichain
• Reactivity: Human
• Synonyms: CTSH (Cathepsin H); Human CTSH (Cathepsin H) ELISA Kit; Pro-cathepsin H; Cathepsin H mini chain; Cathepsin H; Cathepsin H heavy chain; Cathepsin H light chain; CPSB; CTSH; CTSH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CTSH. No significant cross-reactivity or interference between CTSH and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSH elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 23110955
• NCBI GeneID: 1512
• NCBI Accession #: NP_004381.2
• NCBI GenBank Nucleotide #: NM_004390.3
• UniProt Accession #: P09668; Q96NY6; Q9BUM7; B2RBK0
• Molecular Weight: 160,000
• NCBI Official Full Name: pro-cathepsin H preproprotein
• NCBI Official Synonym Full Names: cathepsin H
• NCBI Official Symbol: CTSH
• NCBI Official Synonym Symbols: CPSB; ACC-4; ACC-5; minichain
• NCBI Protein Information: pro-cathepsin H; aleurain; cathepsin B3; cathepsin BA; N-benzoylarginine-beta-naphthylamide hydrolase
• UniProt Protein Name: Pro-cathepsin H
• Protein Family: Pro-cathepsin
• UniProt Gene Name: CTSH
• UniProt Synonym Gene Names: CPSB
• UniProt Entry Name: CATH_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. [provided by RefSeq, Mar 2010]

Uniprot Description

CTSH: Important for the overall degradation of proteins in lysosomes. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.16; Protease

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: extracellular space; lysosome; acrosome; outer dense fiber; cytosol

Molecular Function: peptidase activity; protein binding; protein self-association; HLA-A specific activating MHC class I receptor activity; serine-type endopeptidase activity; cysteine-type endopeptidase activity; endopeptidase activity; protein complex binding; caspase activator activity; aminopeptidase activity; kininogen binding; cysteine-type peptidase activity

Biological Process: caspase activation; adaptive immune response; response to retinoic acid; protein destabilization; membrane protein proteolysis; proteolysis; antigen processing and presentation; positive regulation of angiogenesis; positive regulation of cell proliferation; zymogen activation; spermatogenesis; surfactant homeostasis; metanephros development; immune response-regulating signal transduction; positive regulation of cell migration; T cell mediated cytotoxicity; negative regulation of apoptosis