Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Follistatin Like Protein 1 (FSTL1) Standard Curve.)
Human Follistatin Like Protein 1 (FSTL1) ELISA Kit | FSTL1 elisa kit
Human Follistatin Like Protein 1 (FSTL1) ELISA Kit
Gene Names
FSTL1; FRP; FSL1; OCC1; OCC-1; tsc36; MIR198
Reactivity
Human
Synonyms
Follistatin Like Protein 1 (FSTL1); Human Follistatin Like Protein 1 (FSTL1) ELISA Kit; FSTL1; FLJ50214; FLJ52277; FRP; FSL1; follistatin-related protein; FSTL1 elisa kit
Reactivity
Human
Specificity
Human Follistatin Like Protein 1 (FSTL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human FSTL1. No significant cross-reactivity or interference between Human FSTL1 and analogues was observed.
Sequence Length
308
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Follistatin Like Protein 1 (FSTL1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Follistatin Like Protein 1 (FSTL1) Standard Curve.)
Related Product Information for FSTL1 elisa kit
Description: This Human Follistatin Like Protein 1 (FSTL1) ELISA Kit employs a two-site sandwich ELISA to quantitate FSTL1 in samples. An antibody specific for FSTL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSTL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FSTL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSTL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: FSTL1 encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis.FSTL1 encodes a deduced 308-amino acid protein with an N-terminal signal peptide of 20 amino acids. The number and distribution of the cysteine residues supports the existence of several intramolecular disulfide bridges.Immunohistochemical analysis demonstrated increased expression in Cd90 (THY1)-positive fibroblasts, particularly early in CIA. Monkey fibroblasts transfected with human FSTL1 spontaneously secreted more IL6, and human monocytes transfected with FSTL1 and stimulated with mitogens expressed higher IL1B, TNF, and IL6.
Background: FSTL1 encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis.FSTL1 encodes a deduced 308-amino acid protein with an N-terminal signal peptide of 20 amino acids. The number and distribution of the cysteine residues supports the existence of several intramolecular disulfide bridges.Immunohistochemical analysis demonstrated increased expression in Cd90 (THY1)-positive fibroblasts, particularly early in CIA. Monkey fibroblasts transfected with human FSTL1 spontaneously secreted more IL6, and human monocytes transfected with FSTL1 and stimulated with mitogens expressed higher IL1B, TNF, and IL6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
follistatin-related protein 1
NCBI Official Synonym Full Names
follistatin like 1
NCBI Official Symbol
FSTL1
NCBI Official Synonym Symbols
FRP; FSL1; OCC1; OCC-1; tsc36; MIR198
NCBI Protein Information
follistatin-related protein 1
UniProt Protein Name
Follistatin-related protein 1
Protein Family
UniProt Gene Name
FSTL1
UniProt Synonym Gene Names
FRP
UniProt Entry Name
FSTL1_HUMAN
NCBI Description
This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. [provided by RefSeq, Jul 2008]
Uniprot Description
FSTL1: May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3q13.33
Cellular Component: extracellular space; extracellular region
Molecular Function: heparin binding; protein binding; calcium ion binding
Biological Process: BMP signaling pathway; response to starvation
Research Articles on FSTL1
Similar Products
Product Notes
The Human FSTL1 fstl1 (Catalog #AAA9716287) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716287 ELISA Kit recognizes Human FSTL1. It is sometimes possible for the material contained within the vial of "Follistatin Like Protein 1 (FSTL1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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