Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plasmalemma vesicle-associated protein (PLVAP) Standard Curve.)
Human Plasmalemma Vesicle-Associated Protein (PLVAP) ELISA Kit | PLVAP elisa kit
Human Plasmalemma Vesicle-Associated Protein (PLVAP) ELISA Kit
Gene Names
PLVAP; PV1; FELS; PV-1; gp68; DIAR10
Reactivity
Human
Synonyms
Plasmalemma Vesicle-Associated Protein (PLVAP); Human Plasmalemma Vesicle-Associated Protein (PLVAP) ELISA Kit; PLVAP; FELS; PV-1; PV1; gp68; fenestrated-endothelial linked structure protein; PV-1 protein; PLVAP elisa kit
Reactivity
Human
Specificity
Human Plasmalemma vesicle-associated protein (PLVAP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLVAP. No significant cross-reactivity or interference between Human PLVAP and analogues was observed.
Sequence Length
442
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plasmalemma vesicle-associated protein (PLVAP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Plasmalemma vesicle-associated protein (PLVAP) Standard Curve.)
Related Product Information for PLVAP elisa kit
Description: This Human Plasmalemma vesicle-associated protein (PLVAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PLVAP in samples. An antibody specific for PLVAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLVAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLVAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLVAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PLVAP is a type II integral membrane protein with a short N-terminal cytoplasmic tail, a single-span transmembrane domain, and a large C-terminal extracellular domain consisting mostly of alpha helices. It also contains 2 coiled-coil domains predicted to contribute to dimer formation. The extracellular domain has 4 N-glycosylation sites, a proline-rich region, and regular spacing of cysteine residues. The deduced 439-amino acid mouse Plvap protein contains a consensus casein kinase II phosphorylation site that is not found in the human protein. Rat Plvap contains 2 casein kinase II phosphorylation sites. Northern blot analysis revealed a single PLVAP transcript of about 2.4 kb in pancreas, kidney, placenta, muscle, lung, heart, and liver. No signal was detected in brain or testis.
Background: PLVAP is a type II integral membrane protein with a short N-terminal cytoplasmic tail, a single-span transmembrane domain, and a large C-terminal extracellular domain consisting mostly of alpha helices. It also contains 2 coiled-coil domains predicted to contribute to dimer formation. The extracellular domain has 4 N-glycosylation sites, a proline-rich region, and regular spacing of cysteine residues. The deduced 439-amino acid mouse Plvap protein contains a consensus casein kinase II phosphorylation site that is not found in the human protein. Rat Plvap contains 2 casein kinase II phosphorylation sites. Northern blot analysis revealed a single PLVAP transcript of about 2.4 kb in pancreas, kidney, placenta, muscle, lung, heart, and liver. No signal was detected in brain or testis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
plasmalemma vesicle-associated protein
NCBI Official Synonym Full Names
plasmalemma vesicle associated protein
NCBI Official Symbol
PLVAP
NCBI Official Synonym Symbols
PV1; FELS; PV-1; gp68; DIAR10
NCBI Protein Information
plasmalemma vesicle-associated protein
UniProt Protein Name
Plasmalemma vesicle-associated protein
Protein Family
UniProt Gene Name
PLVAP
UniProt Synonym Gene Names
FELS; PV1; PV-1
Uniprot Description
Involved in the formation of stomatal and fenestral diaphragms of caveolae. May function in microvascular permeability.
Research Articles on PLVAP
1. PV-1 transcription was markedly induced in diabetic retina and by VEGF in retinal endothelial cells
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Product Notes
The Human PLVAP plvap (Catalog #AAA9716019) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716019 ELISA Kit recognizes Human PLVAP. It is sometimes possible for the material contained within the vial of "Plasmalemma Vesicle-Associated Protein (PLVAP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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