Human Plasmalemma Vesicle Associated Protein (PLVAP) ELISA Kit | PLVAP elisa kit

Human Plasmalemma Vesicle Associated Protein (PLVAP) ELISA Kit

Cat. Num. AAA2023863

• Gene Names: PLVAP; PV1; FELS; PV-1; gp68
• Reactivity: Human
• Synonyms: Plasmalemma Vesicle Associated Protein (PLVAP); Human Plasmalemma Vesicle Associated Protein (PLVAP) ELISA Kit; FELS; PV-1; PV1; gp68; Fenestrated-Endothelial Linked Structure Protein; Plasmalemma vesicle protein 1; PLVAP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PLVAP. No significant cross-reactivity or interference between PLVAP and analogues was observed.
• Sequence Length: 442

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.060ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PLVAP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PLVAP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLVAP elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PLVAP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PLVAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLVAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLVAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PLVAP elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 13775238
• NCBI GeneID: 83483
• NCBI Accession #: NP_112600.1
• NCBI GenBank Nucleotide #: NM_031310.2
• UniProt Accession #: Q9BX97; Q86VP0; Q8N8Y0; Q8ND68; Q8TER8; Q9BZD5
• Molecular Weight: 50,594 Da
• NCBI Official Full Name: plasmalemma vesicle-associated protein
• NCBI Official Synonym Full Names: plasmalemma vesicle associated protein
• NCBI Official Symbol: PLVAP
• NCBI Official Synonym Symbols: PV1; FELS; PV-1; gp68
• NCBI Protein Information: plasmalemma vesicle-associated protein
• UniProt Protein Name: Plasmalemma vesicle-associated protein
• Protein Family: Plasmalemma vesicle-associated protein
• UniProt Gene Name: PLVAP
• UniProt Synonym Gene Names: FELS; PV1; PV-1
• UniProt Entry Name: PLVAP_HUMAN

Uniprot Description

PLVAP: Involved in the formation of stomatal and fenestral diaphragms of caveolae. May function in microvascular permeability.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cell surface; perinuclear region of cytoplasm; integral to membrane; caveola

Molecular Function: protein binding; protein homodimerization activity

Biological Process: positive regulation of cellular extravasation; tumor necrosis factor-mediated signaling pathway; MAPKKK cascade