Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DnaJ homolog subfamily C member 4 (DNAJC4) Standard Curve.)
Human DnaJ Homolog Subfamily C Member 4 (DNAJC4) ELISA Kit | DNAJC4 elisa kit
Human DnaJ Homolog Subfamily C Member 4 (DNAJC4) ELISA Kit
Gene Names
DNAJC4; HSPF2; MCG18; DANJC4
Reactivity
Human
Synonyms
DnaJ Homolog Subfamily C Member 4 (DNAJC4); Human DnaJ Homolog Subfamily C Member 4 (DNAJC4) ELISA Kit; DNAJC4; DANJC4; HSPF2; MCG18; MGC19482; MGC57189; MGC71863; heat shock 40kD protein 2; DNAJC4 elisa kit
Reactivity
Human
Specificity
Human DnaJ homolog subfamily C member 4 (DNAJC4) ELISA Kit has high sensitivity and excellent specificity for detection of Human DNAJC4. No significant cross-reactivity or interference between Human DNAJC4 and analogues was observed.
Sequence Length
249
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DnaJ homolog subfamily C member 4 (DNAJC4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DnaJ homolog subfamily C member 4 (DNAJC4) Standard Curve.)
Related Product Information for DNAJC4 elisa kit
Description: This Human DnaJ homolog subfamily C member 4 (DNAJC4) ELISA Kit employs a two-site sandwich ELISA to quantitate DNAJC4 in samples. An antibody specific for DNAJC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DNAJC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNAJC4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNAJC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The E. coli heat-shock protein DnaJ is the founding member of a family of proteins that are associated with protein folding, complex assembly, and export. Prokaryotic and eukaryotic DnaJ homologs contain an approximately 70-amino acid J domain, which is thought to mediate interaction with 70-kD heat-shock proteins. The HSPF2 gene is located approximately 250 bp centromeric of the VEGFB gene. The HSPF2 coding sequence is contained in 5 exons. The deduced 241-amino acid HSPF2 protein has a J domain followed by a predicted transmembrane domain. The human and mouse HSPF2 proteins are 71% identical. Northern blot analysis detected an approximately 1.4-kb HSPF2 transcript in all human cell lines examined.
Background: The E. coli heat-shock protein DnaJ is the founding member of a family of proteins that are associated with protein folding, complex assembly, and export. Prokaryotic and eukaryotic DnaJ homologs contain an approximately 70-amino acid J domain, which is thought to mediate interaction with 70-kD heat-shock proteins. The HSPF2 gene is located approximately 250 bp centromeric of the VEGFB gene. The HSPF2 coding sequence is contained in 5 exons. The deduced 241-amino acid HSPF2 protein has a J domain followed by a predicted transmembrane domain. The human and mouse HSPF2 proteins are 71% identical. Northern blot analysis detected an approximately 1.4-kb HSPF2 transcript in all human cell lines examined.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dnaJ homolog subfamily C member 4 isoform 1
NCBI Official Synonym Full Names
DnaJ heat shock protein family (Hsp40) member C4
NCBI Official Symbol
DNAJC4
NCBI Official Synonym Symbols
HSPF2; MCG18; DANJC4
NCBI Protein Information
dnaJ homolog subfamily C member 4
UniProt Protein Name
DnaJ homolog subfamily C member 4
Protein Family
UniProt Gene Name
DNAJC4
UniProt Synonym Gene Names
HSPF2; MCG18
Research Articles on DNAJC4
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Product Notes
The Human DNAJC4 dnajc4 (Catalog #AAA9715662) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715662 ELISA Kit recognizes Human DNAJC4. It is sometimes possible for the material contained within the vial of "DnaJ Homolog Subfamily C Member 4 (DNAJC4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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