Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DnaJ homolog subfamily C member 12 (DNAJC12) Standard Curve.)
Human DnaJ Homolog Subfamily C Member 12 (DNAJC12) ELISA Kit | DNAJC12 elisa kit
Human DnaJ Homolog Subfamily C Member 12 (DNAJC12) ELISA Kit
Gene Names
DNAJC12; JDP1; HPANBH4
Reactivity
Human
Synonyms
DnaJ Homolog Subfamily C Member 12 (DNAJC12); Human DnaJ Homolog Subfamily C Member 12 (DNAJC12) ELISA Kit; DNAJC12; RP11-57G10.2; JDP1; J domain containing protein 1; J domain containing protein 1 (JDP1); J domain protein 1; DNAJC12 elisa kit
Reactivity
Human
Specificity
Human DnaJ homolog subfamily C member 12 (DNAJC12) ELISA Kit has high sensitivity and excellent specificity for detection of Human DNAJC12. No significant cross-reactivity or interference between Human DNAJC12 and analogues was observed.
Sequence Length
198
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DnaJ homolog subfamily C member 12 (DNAJC12) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human DnaJ homolog subfamily C member 12 (DNAJC12) Standard Curve.)
Related Product Information for DNAJC12 elisa kit
Description: This Human DnaJ homolog subfamily C member 12 (DNAJC12) ELISA Kit employs a two-site sandwich ELISA to quantitate DNAJC12 in samples. An antibody specific for DNAJC12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DNAJC12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNAJC12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNAJC12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Highly conserved C terminus, but lack the G/F-rich and CRR domains. Hahn et al. (1999) identified a J domain-containing protein in mouse and Drosophila. By database searching for sequences homologous to these proteins, Lee et al. (2000) identified a human homolog, which they designated JDP1, that has 2 isoforms generated by alternative use of polyadenylation sites. The primary isoform is 1.2 kb long and encodes a 198-amino acid protein; the other is 0.7 kb long and encodes a 107-amino acid protein. The 198-amino acid protein shares approximately 77% sequence identity with the mouse protein. Northern blot analysis detected a single transcript of 1.3 kb at high levels in brain, heart, and testis, and at reduced levels in kidney and stomach. In mouse and Drosophila, expression is highest in kidney and restricted to heads, respectively.
Background: Highly conserved C terminus, but lack the G/F-rich and CRR domains. Hahn et al. (1999) identified a J domain-containing protein in mouse and Drosophila. By database searching for sequences homologous to these proteins, Lee et al. (2000) identified a human homolog, which they designated JDP1, that has 2 isoforms generated by alternative use of polyadenylation sites. The primary isoform is 1.2 kb long and encodes a 198-amino acid protein; the other is 0.7 kb long and encodes a 107-amino acid protein. The 198-amino acid protein shares approximately 77% sequence identity with the mouse protein. Northern blot analysis detected a single transcript of 1.3 kb at high levels in brain, heart, and testis, and at reduced levels in kidney and stomach. In mouse and Drosophila, expression is highest in kidney and restricted to heads, respectively.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dnaJ homolog subfamily C member 12 isoform a
NCBI Official Synonym Full Names
DnaJ heat shock protein family (Hsp40) member C12
NCBI Official Symbol
DNAJC12
NCBI Official Synonym Symbols
JDP1; HPANBH4
NCBI Protein Information
dnaJ homolog subfamily C member 12
UniProt Protein Name
DnaJ homolog subfamily C member 12
Protein Family
UniProt Gene Name
DNAJC12
UniProt Synonym Gene Names
JDP1
UniProt Entry Name
DJC12_HUMAN
NCBI Description
This gene encodes a member of a subclass of the HSP40/DnaJ protein family. Members of this family of proteins are associated with complex assembly, protein folding, and export. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
DNAJC12: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Chaperone
Chromosomal Location of Human Ortholog: 10q22.1
Research Articles on DNAJC12
Similar Products
Product Notes
The Human DNAJC12 dnajc12 (Catalog #AAA9715663) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715663 ELISA Kit recognizes Human DNAJC12. It is sometimes possible for the material contained within the vial of "DnaJ Homolog Subfamily C Member 12 (DNAJC12), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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