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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Epidermal growth factor-like protein 6 (EGFL6) Standard Curve.)

Human Epidermal Growth Factor-Like Protein 6 (EGFL6) ELISA Kit | EGFL6 elisa kit

Human Epidermal Growth Factor-Like Protein 6 (EGFL6) ELISA Kit

Gene Names
EGFL6; W80; MAEG
Reactivity
Human
Synonyms
Epidermal Growth Factor-Like Protein 6 (EGFL6); Human Epidermal Growth Factor-Like Protein 6 (EGFL6) ELISA Kit; EGFL6; DKFZp564P2063; MAEG; W80; EGF repeat-containing protein 6; MAM and EGF domain containing; epidermal growth factor-like protein 6; EGFL6 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Epidermal growth factor-like protein 6 (EGFL6) ELISA Kit has high sensitivity and excellent specificity for detection of Human EGFL6. No significant cross-reactivity or interference between Human EGFL6 and analogues was observed.
Sequence Length
553
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Epidermal growth factor-like protein 6 (EGFL6) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Epidermal growth factor-like protein 6 (EGFL6) Standard Curve.)
Related Product Information for EGFL6 elisa kit
Description: This Human Epidermal growth factor-like protein 6 (EGFL6) ELISA Kit employs a two-site sandwich ELISA to quantitate EGFL6 in samples. An antibody specific for EGFL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGFL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFL6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: EGFL6 encodes a member of the epidermal growth factor (EGF) repeat superfamily.The predicted 553-amino acid EGFL6 protein has a putative N-terminal signal peptide, which suggests that it is secreted; an EGF repeat region containing 4 complete EGF-like repeats and 1 partial EGF-like repeat; an integrin association motif (RGD); 2 potential N-glycosylation sites; and a potential tyrosine phosphorylation site. Northern blot analysis of a variety of normal human tissues detected an approximately 2.4-kb EGFL6 transcript only in placenta. Among the cancer tissues tested, EGFL6 expression was found only in meningioma tumors. Screening-by-hybridization analysis of various cDNA libraries indicated EGFL6 expression in lung tumor, fetal lung, fetal skin, fetal umbilical cord, fetal liver/spleen, and placenta, but not in normal adult tissues, including lung.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
epidermal growth factor-like protein 6 isoform 1
NCBI Official Synonym Full Names
EGF like domain multiple 6
NCBI Official Symbol
EGFL6
NCBI Official Synonym Symbols
W80; MAEG
NCBI Protein Information
epidermal growth factor-like protein 6
UniProt Protein Name
Epidermal growth factor-like protein 6
UniProt Gene Name
EGFL6
UniProt Synonym Gene Names
MAEG; EGF-like protein 6
UniProt Entry Name
EGFL6_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle, proliferation, and developmental processes. The gene product contains a signal peptide, suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat, 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain, which is believed to have an adhesive function. This gene is expressed early during development, and its expression has been detected in lung and meningioma tumors. [provided by RefSeq, Jul 2008]

Uniprot Description

EGFL6: May bind integrin alpha-8/beta-1 and play a role in hair follicle morphogenesis. Promotes matrix assembly. Belongs to the nephronectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: extracellular space; membrane; basement membrane

Molecular Function: integrin binding; calcium ion binding

Biological Process: extracellular matrix organization and biogenesis; multicellular organismal development; cell adhesion; cell cycle; cell differentiation

Research Articles on EGFL6

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Product Notes

The Human EGFL6 egfl6 (Catalog #AAA9715608) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715608 ELISA Kit recognizes Human EGFL6. It is sometimes possible for the material contained within the vial of "Epidermal Growth Factor-Like Protein 6 (EGFL6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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