Human Sorbitol Dehydrogenase (SDH) ELISA Kit | SDH elisa kit

Human Sorbitol Dehydrogenase (SDH) ELISA Kit

Cat. Num. AAA9714223

• Gene Names: SORD; SORD1; HEL-S-95n
• Reactivity: Human
• Synonyms: Sorbitol Dehydrogenase (SDH); Human Sorbitol Dehydrogenase (SDH) ELISA Kit; SORD; SORD1; SDH elisa kit

• Reactivity: Human
• Specificity: Human Sorbitol dehydrogenase (SDH) ELISA Kit has high sensitivity and excellent specificity for detection of Human SORD. No significant cross-reactivity or interference between Human SORD and analogues was observed.
• Sequence Length: 357

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Sorbitol dehydrogenase (SDH) Standard Curve.)

Related Product Information for SDH elisa kit

Description: This Human Sorbitol dehydrogenase (SDH) ELISA Kit employs a two-site sandwich ELISA to quantitate SORD in samples. An antibody specific for SORD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SORD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SORD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SORD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.

NCBI and Uniprot Product Information

• NCBI GI #: 292495088
• NCBI GeneID: 6652
• UniProt Accession #: Q00796
• NCBI Official Full Name: Sorbitol dehydrogenase
• NCBI Official Synonym Full Names: sorbitol dehydrogenase
• NCBI Official Symbol: SORD
• NCBI Official Synonym Symbols: SORD1; HEL-S-95n
• NCBI Protein Information: sorbitol dehydrogenase
• UniProt Protein Name: Sorbitol dehydrogenase
• Protein Family: Serine 3-dehydrogenase
• UniProt Gene Name: SORD
• UniProt Entry Name: DHSO_HUMAN

NCBI Description

Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]

Uniprot Description

SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family.

Protein type: EC 1.1.1.14; Oxidoreductase; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: extracellular space; membrane; mitochondrial membrane

Molecular Function: zinc ion binding; L-iditol 2-dehydrogenase activity; carbohydrate binding; NAD binding

Biological Process: sperm motility; L-xylitol metabolic process; L-xylitol catabolic process; glucose metabolic process; fructose biosynthetic process; sorbitol catabolic process