Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Mitochondrial-processing peptidase subunit beta (PMPCB) Standard Curve.)
Rat Mitochondrial-Processing Peptidase Subunit beta (PMPCB) ELISA Kit | PMPCB elisa kit
Rat Mitochondrial-Processing Peptidase Subunit beta (PMPCB) ELISA Kit
Gene Names
Pmpcb; Mppb
Reactivity
Rat
Synonyms
Mitochondrial-Processing Peptidase Subunit beta (PMPCB); Rat Mitochondrial-Processing Peptidase Subunit beta (PMPCB) ELISA Kit; PMPCB; Beta-MPP; MPP11; MPPB; MPPP52; mitochondrial processing peptidase beta subunit; PMPCB elisa kit
Reactivity
Rat
Specificity
Rat Mitochondrial-processing peptidase subunit beta (PMPCB) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PMPCB. No significant cross-reactivity or interference between Rat PMPCB and analogues was observed.
Sequence Length
489
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Mitochondrial-processing peptidase subunit beta (PMPCB) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Mitochondrial-processing peptidase subunit beta (PMPCB) Standard Curve.)
Related Product Information for PMPCB elisa kit
Description: This Rat Mitochondrial-processing peptidase subunit beta (PMPCB) ELISA Kit employs a two-site sandwich ELISA to quantitate PMPCB in samples. An antibody specific for PMPCB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PMPCB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PMPCB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PMPCB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PMPCb is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.In in vitro assays, MPPB bound frataxin, which was cleaved by the reconstituted MPP heterodimer. MPP cleavage of frataxin resulted in an intermediate form, comprising amino acids 41 to 210, which was processed further to the mature form. In vitro and in vivo experiments suggested that 2 C-terminal missense mutations found in FRDA patients, I151F and G130V, modulated interaction with MPP-beta, resulting in a slower maturation process at the normal cleavage site.
Background: PMPCb is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.In in vitro assays, MPPB bound frataxin, which was cleaved by the reconstituted MPP heterodimer. MPP cleavage of frataxin resulted in an intermediate form, comprising amino acids 41 to 210, which was processed further to the mature form. In vitro and in vivo experiments suggested that 2 C-terminal missense mutations found in FRDA patients, I151F and G130V, modulated interaction with MPP-beta, resulting in a slower maturation process at the normal cleavage site.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mitochondrial-processing peptidase subunit beta
NCBI Official Synonym Full Names
peptidase, mitochondrial processing beta subunit
NCBI Official Symbol
Pmpcb
NCBI Official Synonym Symbols
Mppb
NCBI Protein Information
mitochondrial-processing peptidase subunit beta
UniProt Protein Name
Mitochondrial-processing peptidase subunit beta
UniProt Gene Name
Pmpcb
UniProt Synonym Gene Names
Mppb
UniProt Entry Name
MPPB_RAT
NCBI Description
beta subunit of mitochondrial processing peptidase, which catalyzes cleavage of the amino terminal leader peptide of nuclear encoded mitochondrial proteins [RGD, Feb 2006]
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Product Notes
The Rat PMPCB pmpcb (Catalog #AAA9712555) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712555 ELISA Kit recognizes Rat PMPCB. It is sometimes possible for the material contained within the vial of "Mitochondrial-Processing Peptidase Subunit beta (PMPCB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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