Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Deleted In Liver Cancer 1 (DLC1) Standard Curve.)
Rat Deleted In Liver Cancer 1 (DLC1) ELISA Kit | DLC1 elisa kit
Rat Deleted In Liver Cancer 1 (DLC1) ELISA Kit
Gene Names
Dlc1; RhoGAP; Arhgap7
Reactivity
Rat
Synonyms
Deleted In Liver Cancer 1 (DLC1); Rat Deleted In Liver Cancer 1 (DLC1) ELISA Kit; DLC1; ARHGAP7; FLJ21120; HP; STARD12; p122-RhoGAP; Rho-GTPase-activating protein 7; StAR-related lipid transfer (START) domain containing 12; deleted in liver cancer 1 variant 2; DLC1 elisa kit
Reactivity
Rat
Specificity
Rat Deleted In Liver Cancer 1 (DLC1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DLC1. No significant cross-reactivity or interference between Rat DLC1 and analogues was observed.
Sequence Length
1091
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Deleted In Liver Cancer 1 (DLC1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Deleted In Liver Cancer 1 (DLC1) Standard Curve.)
Related Product Information for DLC1 elisa kit
Description: This Rat Deleted In Liver Cancer 1 (DLC1) ELISA Kit employs a two-site sandwich ELISA to quantitate DLC1 in samples. An antibody specific for DLC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Deleted in Liver Cancer 1 is deleted in the primary tumor of hepatocellular carcinoma. It maps to 8p22-p21.3, a region frequently deleted in solid tumors. It is suggested that this gene is a candidate tumor suppressor gene for human liver cancer, as well as for prostate, lung, colorectal, and breast cancers.DLC1 is frequently inactivated in human hepatocellular carcinoma, as well as some nasopharyngeal, lung, breast, prostate, kidney, colon, uterine, ovarian, and gastric cancers. The DLC1 protein contains four major functional domains: an N-terminal sterile alpha motif (SAM), a serine-rich (SR) region, a Rho-GAP domain, and a C-terminal steroidogenic acute regulatory protein related lipid-transfer (START) domain. DLC1 is localized to focal adhesions located at the periphery of cells.
Background: Deleted in Liver Cancer 1 is deleted in the primary tumor of hepatocellular carcinoma. It maps to 8p22-p21.3, a region frequently deleted in solid tumors. It is suggested that this gene is a candidate tumor suppressor gene for human liver cancer, as well as for prostate, lung, colorectal, and breast cancers.DLC1 is frequently inactivated in human hepatocellular carcinoma, as well as some nasopharyngeal, lung, breast, prostate, kidney, colon, uterine, ovarian, and gastric cancers. The DLC1 protein contains four major functional domains: an N-terminal sterile alpha motif (SAM), a serine-rich (SR) region, a Rho-GAP domain, and a C-terminal steroidogenic acute regulatory protein related lipid-transfer (START) domain. DLC1 is localized to focal adhesions located at the periphery of cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Rho GTPase-activating protein 7
NCBI Official Synonym Full Names
DLC1 Rho GTPase activating protein
NCBI Official Symbol
Dlc1
NCBI Official Synonym Symbols
RhoGAP; Arhgap7
NCBI Protein Information
rho GTPase-activating protein 7
UniProt Protein Name
Rho GTPase-activating protein 7
Protein Family
UniProt Gene Name
Dlc1
UniProt Synonym Gene Names
Arhgap7; Rhogap; Stard12; DLC-1; StARD12
UniProt Entry Name
RHG07_RAT
Research Articles on DLC1
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Product Notes
The Rat DLC1 dlc1 (Catalog #AAA9712429) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712429 ELISA Kit recognizes Rat DLC1. It is sometimes possible for the material contained within the vial of "Deleted In Liver Cancer 1 (DLC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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